| 摘要: |
| 【目的】研究日粮不同能量水平对獭兔免疫性能及IFN-γ表达量的影响，为獭兔饲养能量标准的制定提供科学依据。【方法】将160只1.5月龄獭兔随机分为Ⅰ、Ⅱ、Ⅲ、Ⅳ4组，每组40只，分别饲喂消化能为9.7，10.5，11.3和12.1 MJ/kg的4种日粮（各日粮其他营养成分一致）；预试期7 d，正试期2个月；每个月采样1次，进行相关免疫指标的测定。【结果】在日粮能量水平为9.7～11.3 MJ/kg时，獭兔的免疫器官指数、免疫球蛋白含量以及肝脏IFN-γ表达量均随日粮能量水平的升高呈现出一定的规律性。2.5月龄时，Ⅰ组与Ⅳ组肝脏指数、脾脏指数、IgG含量以及肝脏IFN-γ表达量的差异均达到了显著水平（P<0.05）。3.5月龄时，Ⅰ组与Ⅲ组的脾脏指数、IgG和SIgA含量及Ⅰ组与Ⅳ组的肝脏IFN-γ表达量均差异显著（P<0.05）。【结论】在日粮能量水平为9.7～11.3 MJ/kg时，日粮能量水平的升高能够增大獭兔的免疫器官指数，促进獭兔免疫球蛋白的分泌以及肝脏IFN-γ的表达,獭兔日粮较适宜的消化能水平为11.3 MJ/kg。 |
| 关键词: 獭兔 日粮 能量水平 免疫性能 IFN-γ表达量 |
| DOI： |
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| 基金项目:陕西省农业科技攻关项目(2010K01-16) |
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| Effect of diet energy level on immune function and expression of hepatic IFN-γ gene of 1.5-3.5 months old rex rabbits |
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CHANG Wan-bo,REN Zhan-jun,HAN Ying,et al
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| Abstract: |
| 【Objective】The experiment was conducted to investigate the effect of diet energy level on immune function and hepatic IFN-γ expression of rex rabbits.【Method】160 weaned rex rabbits with age of 1.5 months were randomly divided into Ⅰ,Ⅱ,Ⅲ and Ⅳ 4 groups with 4 replicates per group and 10 rabbits per replicate.DE values of diets fed to rabbits were 9.7(Group Ⅰ),10.5(Group Ⅱ),11.3(Group Ⅲ),and 12.1(Group Ⅳ) MJ/kg,respectively.Other nutritional ingredients were kept consistent.The experiment included a pretrial period of 7 days and an experimental period of 2 months.Samples were collected every month to measure immune indices.【Result】Immune organ index,immune globulin content and expression of hepatic IFN-γ changed as the energy levels increased from 9.7 to 11.3 MJ/kg.Group Ⅰ and Group Ⅳ had significant difference in hepatic index,IgG content,SIgA content and expression of hepatic IFN-γ when the age was 2.5 months (P<0.05).Group Ⅰ and Group Ⅲ had significant difference in spleen index,IgG content,SIgA content and expression of hepatic IFN-γ when the age was 3.5 months (P<0.05).【Conclusion】Increasing diet energy level in a certain range could enhance the immunity of rex rabbits.The suggested diet digestible energy level was 11.3 MJ/kg. |
| Key words: rex rabbits diet energy level immune function expression of IFN-γ |
