›› 2015, Vol. 42 ›› Issue (10): 2772-2778.doi: 10.16431/j.cnki.1671-7236.2015.10.038

• 疾病防治 • 上一篇    下一篇

断奶仔猪腹泻病因分析

郑龙龙1, 朱玲云1, 陈关雄1,2, 刘萍丹1, 唐宁1, 赵德育1, 刘佳1, 杨贵树1, 白卫兵1   

  1. 1. 云南农业大学动物科学技术学院, 昆明 650201;
    2. 石林彝族自治县畜牧兽医总站, 石林 652200
  • 收稿日期:2015-01-06 出版日期:2015-10-20 发布日期:2015-10-23
  • 通讯作者: 白卫兵 E-mail:360536885@qq.com
  • 作者简介:郑龙龙(1988-),男,山西运城人,硕士生,研究方向:动物传染病学与分子流行病学,E-mail:18314575103@163.com
  • 基金资助:
    国家自然科学基金项目(31160509)——反义LNA-DNA嵌合体抑制蓝耳病病毒增殖作用研究

Causes Analysis of Weaning Piglets Diarrhea

ZHENG Long-long1, ZHU Ling-yun1, CHEN Guan-xiong1,2, LIU Ping-dan1, TANG Ning1, ZHAO De-yu1, LIU Jia1, YANG Gui-shu1, BAI Wei-bing1   

  1. 1. College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China;
    2. Shilin Yi Autonomous County Animal Husbandry and Veterinary Station, Shilin 652200, China
  • Received:2015-01-06 Online:2015-10-20 Published:2015-10-23

摘要: 为了解云南省断奶仔猪腹泻的致病性原因,通过对30个发生断奶仔猪腹泻猪场的210份粪便、60份饮用水,总计270份样品,进行病毒及致病性细菌分析。研究结果表明,致病性大肠杆菌严重污染饮用水,致病性大肠杆菌在饮用水中检出40株,阳性率为66.7%,粪便中检出100株,阳性率47.6%;致病性沙门氏菌在饮用水中检出10株,阳性率为16.7%,粪便中检出24株,阳性率为11.4%,均对亚胺培南敏感。在粪便中猪流行性腹泻病毒(porcine epidemic diarrhea virus,PEDV)、猪圆环病毒2型(porcine circovirous type 2,PCV2)、猪伪狂犬病病毒(pseudorabies virus,PRV)、猪瘟病毒(classical swine fever virus,CSFV)、猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)及猪轮状病毒(porcine rotavirus,PoRV)的PCR扩增阳性率为5.2%~13.0%,在饮用水中未检出。通过对云南省断奶仔猪腹泻进行病因分析,了解断奶仔猪腹泻的具体原因,本试验结果为从病因入手防制该病提供更合理、更切合实际情况的理论依据与试验参考。

关键词: 断奶仔猪腹泻; 病毒性腹泻; 细菌性腹泻; 致病性; 肠毒素

Abstract: To understand the pathogenic causes of weaning diarrhea in Yunnan province,we collected 210 feces and 60 drinking water,a total of 270 samples in 30 farms with weaning diarrhea and analyzed the virus and pathogenic bacteria.The results of the study showed that drinking water was sever polluted by pathogenic Escherichia coli,40 strains of pathogenic Escherichia coli were detected in drinking water,the positive rate was 66.7%,and 100 strains in feces with positive rate of 47.6%;10 strains of pathogenic Salmonella were detected in drinking water,the positive rate was 16.7%,and 24 strains in feces with positive rate of 11.4%,and were sensitive to imipenem.PCR amplified positive rates of porcine epidemic diarrhea virus (PEDV),porcine circovirous type 2 (PCV2),pseudorabies virus (PRV),classical swine fever virus (CSFV),porcine reproductive and respiratory syndrome virus (PRRSV) and porcine rotavirus (PoRV) in feces were 5.2% to 13.0%,and they were not detected in drinking water.By analyzing the causes of diarrhea in Yunnan province in weaned piglets analysis,understanding the specific causes of diarrhea in weaned piglets,the results provided a more reasonable and more realistic theoretical basis and experimental reference for prevention and control of the disease from the cause.

Key words: weaning piglets diarrhea; viral diarrhea; bacterial diarrhea; pathogenicity; enterotoxin

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