| 多粘芽孢杆菌Dw 6菌株培养条件的优化及其发酵液抑菌活性的初步研究 |
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| 引用本文：文凤云，王国永，李晓丽，董淑丽，孙 平，李文巧.多粘芽孢杆菌Dw 6菌株培养条件的优化及其发酵液抑菌活性的初步研究[J].西北农业学报,2011,20(4):30~34 |
| DOI:10.7606/j.issn.1004-1389.2011.04.007 |
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| 基金项目:河南科技大学青年科学基金资助项目（2009QN0004）。 |
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| 中文摘要:10倍稀释法筛选出最佳产抑菌活性物质的多粘类芽孢杆菌Dw 6菌株，试验表明Dw 6菌株最佳培养基为发酵培养基。利用4因素均匀设计方案进行培养基配方的优选，同时进行培养时间和发酵条件的优化。结果表明，Dw 6菌株优化后的培养基配方为（50 mL）蛋白胨3.83 g、酵母浸出粉3.9 g、MgSO4·7H2O 0.105 g、K2HPO4·3H2O 0.17 g，最佳培养时间48 h，初始pH 7.0，培养温度30 ℃。经培养基优化后，Dw 6菌株在培养基初始pH 7.0时，30 ℃培养48 h的发酵上清液对大肠杆菌、金黄色葡萄球菌及出芽短梗霉菌均表现出很强的抑制作用。 |
| 中文关键词:多粘芽孢杆菌 培养条件 发酵液 抑菌活性 |
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| Optimization of Ferment Conditions and Antibacterial Activity Study of Paenibacillus polymyxa Dw 6 |
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| Abstract:Paenibacillus polymyxa Dw 6 strain was selected by 10 fold diluted, and fermentation medium is the best culture medium to Dw 6 to secrete antagonists. Ferment conditions were optimized as follow: bran 3.83 g, powder 3.9 g, MgSO4·7H2O 0.105 g and K2HPO4·3H2O 0.17 g dissolve in 50 mL water, and the optimum time is 48h, culture medium original pH 7.0, at 30 ℃.The fermentation broth of Dw 6 has strong activity against E.coli, Staph.aureus and Aureobasidium pullulans after cultured 48 h at 30 ℃ with fermentation medium original pH 7.0. |
| keywords:Paenibacillus polymyxa Ferment condition Fermentation broth Antibacterial activity |
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