二种不同生长性状线纹海马(Hippocampus erectus)的生长、消化和免疫酶活性的研究
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作者单位:

1.集美大学水产学院 福建厦门;2.厦门市海洋职业技术学院

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中图分类号:

S965.3

基金项目:

福建省科技厅产学研合作项目(2017N51012)资助


The study on the growth, digestive enzyme and immune enzyme activities in the two growth status of seahorse Hippocampus erectus
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Fishery College of Jimei University, Fujian, Xiamen

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    摘要:

    (目的)为研究生长缓慢海马出现时间和生理特性,(方法)选取饲养110d正常生长海马(8.57±0.61cm,1.51±0.38g,)与生长缓慢海马(6.68±0.35cm, 0.81±0.14g)进行生长、消化和免疫酶活性以及以及RNA/DNA研究。(结果)结果表明当体长大于5.026cm时,正常生长海马的生长速度明显大于生长缓慢的海马,正常生长海马的日均增重率、特定生长率、肥满度和脏体指数均优于生长缓慢海马(P<0.05)。二种生长状态海马的消化酶(蛋白酶、淀粉酶和脂肪酶)活性在不同反应温度(5℃、15℃、25℃、30℃、35℃、45℃和55℃)和pH(2.0、3.0、4.0、5.0、6.0、7.0、8.0和9.0)条件下的变化趋势基本一致,除淀粉酶活性在35~55℃和蛋白酶在5~25℃外,正常海马消化酶均显著高于生长缓慢海马 (P<0.05)。正常生长海马ACP、AKP、T-AOC和SOD活性分别比生长缓慢海马高12.83%、48.21%、44.71%和65.75%,且差异显著(P<0.05)。正常生长海马MDA仅为生长缓慢海马的61.09%,且差异显著(P<0.05)。另外,正常生长RNA/DNA的比值比生长缓慢海马高23.75%(P<0.05)。(结论)二种不同生长状态的海马生长、消化和免疫性能上均存在差。

    Abstract:

    (Objective)(Method)Compare of the growth, the ratio of RNA/DNA,digestive enzyme and related immune enzyme activities in normal growth seahorse(8.57±0.61cm,1.51±0.38g) and slow growth seahorse(6.68±0.35cm, 0.81±0.14g)of Hippocampus erectus were studied at 110d raising, respectively. (Result)The results showed that the average daily gain, rate specific growth, condition factor and viscera index in the normal growth seahorse were significantly higher than that of the slow growth seahorse(P<0.05) , respectively.The normal growth seahorse grew more faster than that of the slow growth seahorse when the body length was 5.026cm. The amylase ,protease and lipase activity in normal growth seahorse were as the same tendency as that of the slow growth seahorse under different reaction temperatures (5 ℃, 15 ℃, 25 ℃, 30 ℃, 35 ℃, 45 ℃ and 55 ℃)and pH (2.0、3.0、4.0、5.0、6.0、7.0、8.0 and 9.0), however, the digestive enzyme activity of the former were much higher than that of the latter except amylase activity at 35~55 and protease activity at 5~25. ℃.The activities of ACP、AKP、T-AOC and SOD in normal growth seahorse were more 12.83%、48.21%、44.71%and 65.75% than that of the slow growth seahorse(P<0.05), respectively.The MDA of the normal growth seahorse was only 61.09% of that in the slow growth seahorse(P<0.05),and the ratio of RNA/DNA in the normal growth seahorse was higher 23.75% than that of the slow growth seahorse(P<0.05). (Conclusion) There were significantly different between in two growth status seahorse in growth, digestive enzyme and related immune enzyme activities.

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引用本文

黄永春,涂晨凌,翁虹雨,林祥日.二种不同生长性状线纹海马(Hippocampus erectus)的生长、消化和免疫酶活性的研究[J].水产学报,2020,44(10):1637~1644

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  • 收稿日期:2017-10-01
  • 最后修改日期:2018-01-16
  • 录用日期:2020-04-26
  • 在线发布日期: 2020-11-06
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