| 干旱胁迫下不同品种小麦4种功能基因的表达模式及相关生理指标分析 |
| 点此下载全文 |
| 引用本文：韩翠英，刘秉焱，刘虎岐.干旱胁迫下不同品种小麦4种功能基因的表达模式及相关生理指标分析[J].西北农业学报,2015,24(10):28~34 |
| DOI:10.7606/j.issn.1004-1389.2015.10.005 |
| 摘要点击次数: 1013 |
| 全文下载次数: 812 |
|
| 基金项目:西北农林科技大学青年学术骨干研究基金(01140302)。 |
|
| 中文摘要:采用实时荧光定量PCR（Real-time PCR）对干旱胁迫下脂质转移蛋白基因( TaLTP1)、膨胀素基因（ TaEXPB23）、水通道蛋白基因（ TaAQP7）和果聚糖6-果糖基转移酶基因（ Ta6-SFT）在4种小麦叶片中的表达情况进行分析；通过测定相对水分质量分数和果聚糖质量分数来判断小麦生长发育状况。结果显示：干旱胁迫48 h内，4种基因在不同抗旱性小麦叶片中的表达模式不同，干旱敏感型小麦中4种基因的相对表达量先上升后下降，恢复到正常水平；干旱耐受型小麦中4种基因的相对表达量先上升后下降，但仍维持较高表达水平；干旱耐受型小麦具有较高的相对水分质量分数和果聚糖累积量。以上结果表明，4种基因参与小麦干旱胁迫应答，4种基因的表达模式可以作为鉴定小麦抗旱的分子指标。本研究可为准确快速地鉴定小麦品种抗旱性提供重要分子依据。 |
| 中文关键词:小麦 干旱胁迫 TaLTP1 TaEXPB23 TaAQP7 Ta6-SFT |
| |
| Expression of Functional Genes and Physiological Indicators Analysis Induced by Drought Stress in Various Wheat (Triticum aestivum L.) |
|
|
| Abstract:Real-time quantitative PCR(RT-PCR) was used to detect relative expression levels of TaLTP1, TaEXPB23, TaAQP7and Ta6-SFT in different varieties of wheat. In addition,relative water mass fraction and fructan mass fraction were analyzed at the same time. Results showed that the expression levels of four functional genes increase notably firstly and then decreased,however,the expression levels of four genes in two drought-tolerant wheat cultivars were always higher than those in two drought-sensitive wheat cultivars under drought stress condition.The drought-tolerant wheat cultivars lost water more slowly and accumulated a greater amount of fructan than the drought-sensitive wheat cultivars under drought stress condition. The results suggest that those four functional genes probably played a role in drought tolerance in wheat. The expression levels of four function genes could be appraisal indices of drought-tolerance of wheat. This study provides a good index to identify drought-resistance cultivar. |
| keywords:Wheat Drought stress TaLTP1 TaEXPB23 TaAQP7 Ta6-SFT |
| 查看全文 查看/发表评论 下载PDF阅读器 |
