| 4个小麦抗旱相关转录因子基因的表达及其与抗旱生理生化指标的相关性 |
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| 引用本文：刘秉焱，韩翠英，刘虎岐.4个小麦抗旱相关转录因子基因的表达及其与抗旱生理生化指标的相关性[J].西北农业学报,2016,25(4):530~532 |
| DOI:10.7606/j.issn.1004-1389.2016.04.008 |
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| 基金项目:西北农林科技大学青年学术骨干研究基金(01140302)。 |
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| 中文摘要:筛选转录因子基因作为小麦抗旱性鉴定指标，丰富和补充小麦抗旱鉴定体系。以4个抗旱性不同的小麦品种为材料，通过实时荧光定量PCR，检测4个抗旱相关转录因子基因（ TaERF1 , TaMYB30, TaNAC69, Wabi5）在不同处理时间的表达，同时分析干旱胁迫下4个抗旱生理生化指标的变化，探究4个转录因子基因的表达与抗旱生理生化指标之间的相关性。结果表明，基因 TaERF1、 TaMYB30、 TaNAC69和 Wabi5的表达都受干旱胁迫的诱导，且在抗旱性不同的小麦品种中的表达模式存在明显差异； 相关性分析表明， TaERF1在各胁迫持续时间点的表达量都与可溶性蛋白和丙二醛（MDA）呈显著或极显著相关，在胁迫处理3 h、24 h和48 h时， TaERF1的表达量与相对含水量（RWC）显著相关， TaMYB30、 TaNAC69和 Wabi5在一定时段的干旱胁迫后的表达量也分别与可溶性糖、可溶性蛋白、RWC和MDA存在显著相关性。说明转录因子基因 TaERF1、 TaMYB30、 TaNAC69和 Wabi5的表达可以作为评估小麦抗旱性的参考指标。 |
| 中文关键词:小麦 干旱抗性 转录因子 抗旱指标 |
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| Correlation between Expression of Drought-related Transcription Factor Genes and Physiological, Biochemical Indexes in Wheat |
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| Abstract:This paper aims at screening transcription factor genes as indexes of identification of wheat drought resistance,enriching and complementing the identification system of current wheat drought resistance . Correlation between expression of transcription factor genes and four physiological and biochemical indexes was investigated under drought stress using four varieties of wheat. The genes include four drought-related transcription factor genes ( TaERF1 , TaMYB30, TaNAC69, Wabi5 ). The results showed that the expression of TaERF1 , TaMYB30, TaNAC69, Wabi5 were induced by drought stress,and the expression profiles were different among the genes between drought tolerant wheat and drought sensitive wheat. Correlation analysis showed that the expression of TaERF1 was significantly correlated to soluble protein and malonaldehyde (MDA) during drought stress,it also correlated to relative water content (RWC) after 3 h,24 h and 48 h of drought. The expression of TaMYB30, TaNAC69, Wabi5 after some certain time also had significant correlation with soluble protein,soluble sugar,RWC and MDA. The results indicated that the expression of TaERF1, TaMYB30, TaNAC69 and Wabi5 could be appraisal indexes of drought-tolerance of wheat. |
| keywords:Wheat Drought-resistance Transcription factor Drought-resistance index |
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