Plot experiments were conducted to study the effects of fertilizer application rates and time on the accumulation,translocation and distribution of nitrogen(N),phosphorus(P)and potassium(K)as well as the tuber yield of potato.The result showed that tuber yield of different treatments were ranged from 33.5 to 38.6 t/hm2,N agronomic efficiency(AE)ranged from 12.3% to 50.3%.The treatment of low N,P with high K had a significantly higher total tuber yield,marketable tuber yield,marketable tuber ratio than the treatment of high N,P with low K（P＜0.05） and the treatment of low N,P,high K while top dressing with N was the best one.There were no differences on the tendency of nutrients accumulation,but significant difference was showed on the nutrients accumulation and distribution in different organs at different growth stages between four treatments.The treatment of low N,P with high K could boost the accumulation and distribution proportion of N,P,and K in tuber while enhance their translocation from vine to tuber.Top dressing N before tuber bulking stage could improve the distribution of nutrients in tuber,but top dressing K based on top dressing N could only improve the ratio of marketable tuber slightly.