| 异源表达菊芋HtHCT基因对受体植物木质素相关生理生化指标的影响 |
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| 引用本文：张新业，苏彦苹，朱 姝，王聪艳，侯晓强，李文静.异源表达菊芋HtHCT基因对受体植物木质素相关生理生化指标的影响[J].西北农业学报,2022,(1):63~71 |
| DOI:10.7606/j.issn.1004-1389.2022.01.008 |
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| 基金项目:河北省高等学校科学技术研究项目（BJ2018044）；廊坊市科技支撑计划项目（2019012003）。 |
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| 中文摘要:羟基肉桂酰辅酶A:莽草酸/奎宁酸羟基肉桂酰转移酶（HCT）是木质素合成过程中的关键酶之一。本研究构建了菊芋HtHCT基因的植物表达载体pCAMBIA1390-HtHCT，分别对拟南芥和本氏烟进行遗传转化，并对转基因植株进行生理生化指标测定。PCR和RT-PCR结果表明，HtHCT基因已经插入到受体植物基因组中，并能进行转录；转基因植株生理生化指标检测表明，HtHCT基因能够调节拟南芥和本氏烟中木质素的含量及组成，并对受体材料的类黄酮合成亦有一定的影响。 |
| 中文关键词:菊芋 HtHCT基因 木质素 类黄酮 功能研究 |
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| Effect of Heterologous Expression of HtHCT Gene from Helianthus tuberosus on Lignin-related Physiological and Biochemical Indicators of Recipient Plants |
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| Abstract:Hydroxycinnamoyl-CoA:shikimate/quinate hydroxycinnamoyltransferase (HCT) is one of the key enzymes involved in lignin synthesis. In this study,the plant expression vector pCAMBIA1390-HtHCT of HtHCT gene from Helianthus tuberosus was constructed,and then the recombinant expression vector was transformed into Arabidopsis thaliana and Nicotiana benthamiana， respectively. At last,physiological and biochemical indicators of transgenic lines and wild type were measured. The PCR and RT-PCR results indicated HtHCT gene had been integrated into the genome of recipient material and transcripted successfully. The analysis of physiological and biochemical indicators results showed that HtHCT could regulate the lignin content and composition in Arabidopsis thaliana and Nicotiana benthamiana. In addition,the flavonoids content of recipient plants was also affected by the introduction of HtHCT. |
| keywords:Helianthus tuberosus HtHCT Lignin Flavonoids Functional analysis |
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