| 传染性胃肠炎病毒陕西分离株S基因主要抗原位点基因的克隆与序列分析 |
| 点此下载全文 |
| 引用本文：穆 杨，丛日华，梁 宁.传染性胃肠炎病毒陕西分离株S基因主要抗原位点基因的克隆与序列分析[J].西北农业学报,2011,20(7):17~21 |
| DOI:10.7606/j.issn.1004-1389.2011.07.005 |
| 摘要点击次数: 271 |
| 全文下载次数: 173 |
|
| 基金项目:陕西省攻关项目（2008K02 05 3）;西北农林科技大学人才基金项目。 |
|
| 中文摘要:根据GenBank公布的猪传染性胃肠炎病毒S基因的序列，设计合成1对特异性引物，通过RT PCR从用细胞增殖的TGEV病毒液中扩增编码S基因B、C抗原位点的基因片段，然后将获得的片段克隆至pMD18 T载体上，构建重组质粒。通过PCR、酶切和测序鉴定重组质粒，将测序结果与GenBank公布的21个相关序列进行多序列比较并绘制进化树。所克隆的TGEV陕西分离株S基因B、C抗原位点基因片段长度为765 bp，与参考毒株的核苷酸同源性为96.1%~99.9%。进化树分析表明，分离株与中国的H株、HN2002株、TS株,美国的Miller M60株、Miller M6株及英国的FS772株亲缘关系较近，与H株的亲缘关系最近。 |
| 中文关键词:猪传染性胃肠炎病毒 S基因 克隆 序列分析 |
| |
| Cloning and Sequencing of Main Antigenic Sites of S Gene of TGEV Shaanxi Isolate |
|
|
| Abstract:Transmissible gastroenteritis is an acute, enteric contagious disease, caused by transmissible gastroenteritis virus. The infection is associated with high morbidity in pigs of all ages and with high mortality in suckling piglets and may cause devastating economic problems all of the world. In this study, one pairs of special primers were designed and synthesized according to S gene sequence of TGEV in Genbank. The fragment of B,C antigenic site of S gene of TGEV Shaanxi isolate was amplified by RT PCR. Then the fragment was cloned into the pMD18 T vector to construct the reconbination. The reconbination was identificated by PCR, digesting and sequencing. The result of sequencing was compared with 21 correlated series and cladogram was depicted. The results showed that the B,C antigenic site of S gene cloned was 765 bp. Nucleotide homology was 96.1%-99.9% with reference strains. The cladogram analysis showed that the isolate had close relationship with H strain, HN2002 strain, TS strain of China and Miller M60 strain, Miller M6 strain of America and FS772 strain of England. It had the closest relationship with H strain. |
| keywords:TGEV S gene Cloning Sequence analysis |
| 查看全文 查看/发表评论 下载PDF阅读器 |
