三疣梭子蟹细胞Cdk7基因克隆及其在卵巢发育中的表达
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1. 中国水产科学研究院 黄海水产研究所, 农业部海洋渔业可持续发展重点实验室, 山东 青岛 266071;
2. 青岛海洋科学与技术国家实验室, 海洋渔业科学与食物产出过程功能实验室, 山东 青岛 266071

作者简介:

贾复龙(1989-),男,硕士研究生,研究方向为海水养殖生物种质资源与工程育种研究.E-mail:1360639373@qq.com

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中图分类号:

S917

基金项目:

国家自然科学基金项目(41576147,41306178);泰山领军人才工程高校生态农业创新类计划项目(LJNY2015002).


Cloning and expression analysis of Cdk7, a gene involved in ovarian de-velopment, from swimming crab (Portunus trituberculatus)
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1. Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture;Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;
2. Function Laboratory for Marine Fisheries Science and Food

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    摘要:

    本实验应用RACE技术克隆获得了三疣梭子蟹()周期蛋白依赖性激酶7(Cdk7)基因cDNA序列全长。基因5’和3’非编码区域(UTR)以及开放阅读框的长度分别是23 bp、178 bp和1056 bp,预测编码一个含有351个氨基酸,分子量为39.91 kD的蛋白质,包含一个丝氨酸/苏氨酸催化保守结构域和T-loop结构。同源性分析表明,三疣梭子蟹CDK7氨基酸序列与其他物种Cdk7相似度较高,说明<0.05)。<0.05)。去除眼柄后该基因在卵巢组织中的表达呈现先升高后降低的趋势,并于第4天达到最大值。本研究的结果表明,基因参与了三疣梭子蟹卵巢发育调控,为深入开展三疣梭子蟹和其他甲壳动物性腺发育调控机理研究提供了重要信息。

    Abstract:

    The full-length cDNA encoding ) using the rapid amplification of cDNA ends technique (RACE). The complete cDNA was 1257 bp long, and contained an open reading frame (ORF) of 1056 bp, a 23-bp 3' untranslated region, and a 178-bp 5' untranslated region. The ORF encoded a polypeptide of 351 amino acids containing a serine/threonine kinase domain and a T-loop. An homology analysis revealed that the Cdk7 of had high identity with those from other species. Quantitative real-time PCR analyses showed that transcripts were present in all of the tissues examined, with the highest transcript levels in the ovary. The high transcript level of in the ovary was consistent with its role in regulating cell division. The mRNA levels in oogonia were higher in the first and the second stages than in the other stages of overy development, indicating that may play an important role in oogonial mitosis. After eyestalk ablation, the transcript levels of significantly increased in the ovary to peak on day 4, and then decreased. Together, these results suggested that Cdk7 may play important roles in ovarian development, especially oogenesis, in . These data provide useful information for further research on the regulation of gonad development in this species and other crustaceans.

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贾复龙,孟宪亮,刘萍,李健,高保全.三疣梭子蟹细胞Cdk7基因克隆及其在卵巢发育中的表达[J].中国水产科学,2016,23(5):1032-1040
JIA Fulong, MENG Xianliang, LIU Ping, LI Jian, GAO Baoquan. Cloning and expression analysis of Cdk7, a gene involved in ovarian de-velopment, from swimming crab (Portunus trituberculatus)[J]. Journal of Fishery Sciences of China,2016,23(5):1032-1040

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  • 在线发布日期: 2016-09-05
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