1株猪源H9N2亚型流感病毒的分子特征

doi:10.11843/j.issn.0366-6964.2020.09.029

摘要/Abstract

摘要： 旨在进一步了解山东省猪流感的流行情况及其病原特征，笔者于2019年春季，在山东省泰安某屠宰场采集130份猪鼻拭子，进行病毒分离鉴定；并对分离病毒进行全基因组测序和分子特征分析；用禽H9N2亚型标准抗原联合血凝抑制方法检测2018—2019年从山东省8个地区猪场采集的1 527份猪血清样品中的猪流感病毒抗体。结果显示：分离到1株H9N2亚型流感病毒，命名为A/swine/Shandong/TA009/2019（H9N2）。分离病毒与A/environment-air/Kunshan/NIOSH-BL20/2018（H9N2）和A/environment-air/Kunshan/NIOSH-BL25/2018（H9N2）遗传关系最近，其基因片段的核苷酸相似性均在99.5%以上。分离病毒的HA和NA基因属于Y280-like分支，PB2和M基因属于G1-like分支，PB1、PA、NP和NS基因属于SH/F98-like分支。分离病毒HA蛋白裂解位点处的氨基酸序列为“PSRSSR/GL”，符合低致病性禽流感病毒的分子生物学特性。HA蛋白的216位为L，具有结合人源唾液酸α 2，6-Gal的能力。血清学分析结果显示，9份血清中H9N2抗体呈阳性，其总阳性率为0.59%。综上：本研究分离到1株猪源H9N2亚型流感病毒，并在猪血清中检测到H9N2抗体，提示应加强对猪流感的流行情况及其病原特征的持续监测。

关键词: 猪流感病毒, H9N2, 分子特征, 血清学

Abstract: The objective of this study was to explore the epidemic situation and pathogenic characteristics of swine influenza virus (SIV) in Shandong Province. In the spring of 2019, 130 swine nasal swab samples were collected from a slaughterhouse in Tai'an city, Shandong Province for virus isolation and identification. The whole genome of isolated virus was sequenced and analyzed. Meanwhile, 1 527 swine serum samples were collected from swine farms in 8 regions of Shandong province and their anti-SIV antibody were detected by HI assay using standard avian H9N2 antigen. The results showed that a H9N2 subtype influenza virus strain was isolated and named as A/swine/Shandong/TA009/2019(H9N2). The homology analysis showed that the isolated virus had close genetic relationship with A/environment-air/Kunshan/NIOSH-BL20/2018(H9N2) and A/environment-air/Kunshan/NIOSH-BL25/2018(H9N2), and the nucleotide homology of the gene fragments were above 99.5%. Phylogenetic analysis results demonstrated that HA and NA genes of the isolated virus belong to the Y280-like lineage, PB2 and M genes belong to the G1-like lineage, and PB1, PA, NP and NS genes belong to the SH/F98-like lineage. The cleavage site in HA protein is “PSRSSR/GL”, which was in accordance with the molecular biological characteristics of low pathogenic avian influenza virus.The position 216 of HA protein is L, and it has the ability to bind human-derived sialic acid α 2,6-Gal. The results of HI showed that 9 among 1 527 serum samples were positive with a positive rate of 0.59%. The isolated virus was swine-derived H9N2 virus, and serological investigations revealed that H9N2 subtype virus infection was present in swine herds in Shandong Province. The results of this study suggest that continuous surveillance of the SIV epidemiological situation and its pathogenic characteristics should be strengthened.

Key words: swine influenza virus, H9N2, molecular characterization, serology

中图分类号:

S852.659.5

赵玉仲, 丁国飞, 刘家琪, 李莉, 李英超, 王彬, 邵清源, 冯剑, 郭丽红, 刘思当, 肖一红. 1株猪源H9N2亚型流感病毒的分子特征[J]. 畜牧兽医学报, 2020, 51(9): 2312-2318.

ZHAO Yuzhong, DING Guofei, LIU Jiaqi, LI Li, LI Yingchao, WANG Bin, SHAO Qingyuan, FENG Jian, GUO Lihong, LIU Sidang, XIAO Yihong. Molecular Characterization of a H9N2 Subtype Swine Influenza Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(9): 2312-2318.

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