Comparison of the survival rates of different transport modes and temperature for the Pinctada fucata juveniles was made so as to provide references for the transportation. Three-month-old P. fucata juveniles were used as experimental materials, and three methods of dry transport, water transport and oxygen transport were used to simulate transportation at temperature of 5, 13, 21 and 29℃ respectively. One group of experimental juveniles was taken from each group of transport conditions every 3 h, and transferred to a normal environment for temporary recovery, and the survival rate was calculated after 48 h. Nonlinear parameter curve fitting between transportation time and survival rate, and calculated lethal time (T₀), semi-lethal time (T_0.5) and total lethal time (T₁) for each group of transport conditions, two-way analysis of variance were used, and the Duncan method was used to compares the average of the three modes of transport and the four temperatures. The results showed that:the survival rates of oxygen transport mode is higher than the other two transportations, and the water transport is better than dry transport. The survival rates of temperature at 13℃ and 21℃ are highter than that of the other two temperatures, and the temperature from 17.60℃ to 20.05℃ is the optimal temperature for the P. fucata juveniles transportation.