Patinopecten yessoensis including 1F (brown♀× brown♂), 2F(white♀×brown♂), 3F(brown♀×white♂), and 4F(white♀× white♂) were established by inbreeding and crossing. Each family had three parallel groups. Biology parameters of egg size, hatching rate, juvenile’s growth rate and survival percentage were compared among different shell color families. The four families’ genetic diversity was mutually compared with microsatellite markers. Linear regression equations between shell length and culture time, and between shell height and culture time for all lines was obtained. Size of egg diameter, hatching rate of larvae and larval survival rate among 1F, 2F, 3F and 4F families showed no significant differences (>0.05). But daily growth rates of shell length for 1F, 2F, 3F and 4F was significantly different(<0.05), which were 4.349μm/d, respectively. Shell height daily growth rates for 1F, 2F, 3F and 4F were also significantly different from each other(<0.05), which were 4.464μm/drespectively. The growth rates of larva’s shell length and shell height of 4F were significantly higher than those of 1F, 2F, 3F(<0.05). The important genetic parameters of all families such as allele number, expected heterozygosity and observed heterozygosity were analyzed by SSR. The results showed that the 1F family had the lowest genetic diversity.) indicated that the genetic identity between 1F and 4F (0.635 1) was the lowest and the genetic distance between them was the farthest (0.454 0). The present results indicated genetic differentiation exists between white and brown individuals within populations, which caused different larval growth rates among four kinds of shell color families of . The present study provides basic data for selective breeding of different shell’s Japanese scallop.