一株羊源致病性屎肠球菌的分离鉴定及耐药性分析

doi:10.16431/j.cnki.1671-7236.2017.02.032

Abstract

Abstract:

To further identify the pathogenic strains and analyze their antibiotic resistance, the methods of pathogen isolating, the methods of Gram staining, biochemical tests and 16S rRNA PCR amplification, virulence tests, drug sensitive tests, virulence genes and resistance genes PCR amplification were used. The results showed that the strain was Gram-positive and revealed positive after reacting with 6.5% NaCl, melibiose, sucrose and etc., while revealed negative reactions with VP, hippurate, arabinose and etc.. It showed 100% similarity with Enterococcus feacium 16S rRNA gene sequence in GenBank after PCR amplification of 16S rRNA gene and BLAST alignment. It was found severe pathogenicity after virulence tests in mice. The strain was highly resistant to oxacillin, penicillin G of β-lactam antibiotics and norfloxacin, ciprofloxacin, levofloxacin of quinolones antibiotics and tetracycline, while was sensitive to antibiotics of erythromycin, vancomycin and clindamycin. It revealed that virulence factor genes Asal, cylA, acm and resistance genes TetM, ant(6)-Ⅰ, aac(6')-aph (2"), ermB showed positive. The results showed that the bacteria was Enterococcus feacium, it had a strong pathogenicity and severe drug resistance, which might be related to the highly positive rates of virulence genes and drug resistance genes.

Key words: Enterococcus feacium; isolation and identification; drug resistance; virulence factor; resistance gene

CLC Number:

S852.61⁺1

LIU Yong-an, LI Xue-rui, ZHOU Jian-hua, MA Li-na, LIU Yong-sheng. Isolation, Identification and Drug Resistance Analysis of Pathogenic Enterococcus feacium of Sheep[J]. , 2017, 44(2): 530-537.

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Isolation, Identification and Drug Resistance Analysis of Pathogenic Enterococcus feacium of Sheep

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