Abstract: The effects of 2 Bacillus subtilis strains，Q4 and Q10 inhibiting the growth of E. coli and Salmonella.Regarded E.coli and Salmonella as indicators，investigated by the co-incubation， pre-incubation and post-incubation method.The results revealed that 2 Bacillus subtilis strains showed different ability to inhibit the growth of E. coli and Salmonella after incubating with Q4 and Q10， respectively.Having inoculated Bacillus subtilis and then co-incubation pathogens inhibition were most obvious in means of the strongest inhibitory effect at 24 h，at the same time，pre-incubation Bacillus subtilis and then co-incubation pathogen the inhibition was the weakest of all，the pathogens and Bacillus subtilis co-incubation were the second. From the former consequences that 2 Bacillus subtilis strains of Q4 and Q10 had inhibitory effect on the pathogen，but there were differences in inhibition，and related to the duration of Bacillus subtilis and pathogen inoculation.

Key words: Bacillus subtilis; E.coli; Salmonella; bacteriostatic test