Comparative Characterization of Enzymatic Digestion from Fish and Soybean Meal from Simulated Digestive Process of Pacific Bluefin Tuna,Thunnus orientalis |
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| Authors: | Ariana I Román‐Gavilanes Emmanuel Martínez‐Montaño María Teresa Viana |
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| Affiliation: | 1. Programa de Maestría en Biología Molecular y Biotecnología, Facultad de Ciencias Marinas, Universidad Autónoma de Baja California, UABC, Ensenada BC, México;2. Facultad de Ciencias del Mar, Universidad Autónoma de Sinaloa, Mazatlán Sinaloa 82000, México;3. Laboratorio de Nutrición y Fisiología Digestiva, Instituto de Investigaciones Oceanológicas, UABC, Ensenada BC 22860, México |
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| Abstract: | The digestive process of the Pacific bluefin tuna (PBT), Thunnus orientalis, was simulated through two phases of in vitro digestion: acidic digestion with porcine pepsin, followed by alkaline digestion with pancreatic crude extract (PCE) obtained from the PBT to hydrolyze fish meal (FM) and soybean meal (SBM) as protein substrates. The crude protein from FM resulted in a lower degree of hydrolysis (73.3%) compared with SBM (79.2%). However, the resulting digested products showed that FM contained 35% more small peptides, with sizes <6.5 kDa than those from the starting material (>150 kDa). The SBM had an increase of only 1.3% in the similar peptide cut‐offs found after hydrolysis. These results suggested that FM appeared to be a better source of protein according to the amount of low‐molecular weight peptides. In addition, the proteolytic activity of PCE showed that 88.9% of its alkaline proteolytic activity corresponded to trypsin and 2.9% corresponded to chymotrypsin activity. The results shown here demonstrate that peptide sizes are important in identifying suitable protein sources for aquafeed production to reinforce the primary results obtained from the in vitro digestibility using the pH‐Stat system. These results also contribute to a better understanding of the digestibility process in aquatic organisms. |
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