| 杜仲CONSTANS-like全基因组鉴定、系统进化及表达模式分析 |
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| 引用本文： | 刘俊,李龙,陈玉龙,刘燕,吴耀松,任闪闪.杜仲CONSTANS-like全基因组鉴定、系统进化及表达模式分析[J].浙江农林大学学报,2022,39(3):475-485. |
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| 作者姓名： | 刘俊 李龙 陈玉龙 刘燕 吴耀松 任闪闪 |
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| 作者单位： | 1.河南中医药大学 中医药科学院 河南省中医方证信号传导重点实验室，河南 郑州 4500462.国际竹藤中心 国家林业和草原局竹藤科学与技术重点开放实验室，北京 1001023.国际竹藤中心 安徽太平试验中心，安徽 黄山 2457004.西北农林科技大学 林学院，陕西 杨凌 712100 |
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| 基金项目： | 河南省高等学校重点科研项目(22A360005)；国家林业和草原局/北京市共建竹藤科学与技术重点实验室开放基金资助项目(ICBR-2020-05)；国际竹藤中心安徽太平试验中心开放课题(1632021006-4)；河南中医药大学博士科研启动基金项目(RSBSJJ2019-04) |
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| 摘 要： | 目的 揭示CONSTANS-like在杜仲Eucommia ulmoides基因组中的分布、结构特征及表达模式。 方法 利用生物信息学方法，对杜仲CONSTANS-like基因家族进行鉴定及理化性质、进化关系、基因结构、启动子元件和表达模式分析。 结果 杜仲基因组中共鉴定到8个EuCOLs基因，分别命名为EuCOL1~EuCOL8，氨基酸数目为315~469，理论等电点分布范围为5.10~6.47，分子量为35.21~52.65 kDa。亚细胞定位预测均定位在细胞核中，为亲水性蛋白，分布于8条染色体。系统进化分为2个亚家族(群组Ⅰ和群组 Ⅲ)，分别包含2和6个EuCOLs蛋白，同一亚家族基序具有相似性。EuCOLs基因结构简单，启动子中含有多个光周期响应元件。表达模式分析显示:EuCOLs在杜仲叶片发育中表达水平相对较低，EuCOL7在杜仲胶形成中表达量最高，各家族成员表达特征存在差异。蛋白互作预测显示:EuCOL7可与多个光周期响应蛋白互作。 结论 杜仲CONSTANS-like基因家族含有典型的CCT和B-box结构域，可能参与叶片发育及杜仲胶的形成。图8表1参56
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| 关 键 词： | 杜仲 CONSTANS-like 生物信息学 表达模式分析 |
| 收稿时间： | 2021-05-20 |
Genome-wide identification,system evolution and expression pattern analysis of CONSTANS-like in Eucommia ulmoides |
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| LIU Jun,LI Long,CHEN Yulong,LIU Yan,WU Yaosong,REN Shanshan.Genome-wide identification,system evolution and expression pattern analysis of CONSTANS-like in Eucommia ulmoides[J].Journal of Zhejiang A&F University,2022,39(3):475-485. |
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| Authors: | LIU Jun LI Long CHEN Yulong LIU Yan WU Yaosong REN Shanshan |
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| Abstract: | Objective This study aims to reveal the distribution and expression pattern of CONSTANS-like in Eucommia ulmoides genome. Method Bioinformatics method was used to identify the CONSTANS-like gene family of E. ulmoides and analyze its physicochemical properties, evolutionary relationship, gene structure, promoters and expression patterns. Result A total of 8 EuCOL genes (EuCOL1?EuCOL8) were identified in E. ulmoides genome, composed of 315?469 amino acid residues. Their isoelectric point distribution range was 5.10?6.47 and the molecular weight was 35.21?52.65 kDa. Subcellular localization predicted that they were all located in the nucleus and were hydrophilic proteins, distributed on 8 chromosomes. EuCOL gene family was divided into two subfamilies (Group Ⅰ and Group Ⅲ), containing 2 and 6 EuCOLs proteins, respectively, the motifs of the same subfamily were similar. EuCOL genes were simple in structure and contained multiple photoperiodic response elements in their promoters. Expression pattern analysis showed that the expression level of EuCOLs was relatively low during the development of E. ulmoides leaves, the expression level of EuCOL7 was the highest in gutta-percha formation, and the expression characteristics of family members were different. Protein interaction prediction showed that EuCOL7 could interact with multiple photoperiod responsive proteins. Conclusion The CONSTANS-like gene family of E. ulmoides contains a typical CCT and B-box domain, which may be involved in leaf development and gutta-percha formation. Ch, 8 fig. 1 tab. 56 ref.] |
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