| 光皮桦基因组DNA 提取方法比较 |
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| 引用本文： | 谢一青,李志真,黄儒珠,肖祥希,王志洁.光皮桦基因组DNA 提取方法比较[J].浙江农林大学学报,2006,23(6):664-668. |
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| 作者姓名： | 谢一青 李志真 黄儒珠 肖祥希 王志洁 |
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| 作者单位： | 1.福建省林业科学研究院, 福建 福州 350012;2.福建师范大学 生命科学学院, 福建 福州 350007 |
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| 基金项目： | 福建省林业厅重大科学技术攻关项目(200306) |
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| 摘 要： | 为从富含次生代谢物的光皮桦Betula luminifera 嫩叶中获得高质量DNA , 设计了5 种先提核再提DNA 的CTAB 法Ⅰ , CTAB 法Ⅱ , CTAB 法Ⅲ , CTAB 法Ⅳ及改进的SDS 法, 并以常规CTAB 法为对照。用不同的方法就光皮桦基因组DNA 进行了提取, 采用琼脂糖凝胶电泳检测、A260/ A280值测定、限制性内切酶处理和PCR 扩增等方法对所提的DNA 进行了比较分析。结果表明:实验设计的5 种改进方法提取的DNA 质量要比常规法的好, 但提取的效果差异较大。其中改进的CTAB 法Ⅰ是提取光皮桦基因组DNA 的最佳方法;PCR 扩增结果表明, 不同的DNA 提取方法会影响PCR 带型的变化。图3 表1 参13
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| 关 键 词： | 植物学 光皮桦 DNA 提取 CTAB 法 SDS 法 顽拗类植物 |
Comparison of methods of extracting genomic DNA from Betula luminifera |
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| XIE Yi-qing,LI Zhi-zhen,HUANG Ru-zhu,XIAO Xiang-xi,WANG Zhi-jie.Comparison of methods of extracting genomic DNA from Betula luminifera[J].Journal of Zhejiang A&F University,2006,23(6):664-668. |
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| Authors: | XIE Yi-qing LI Zhi-zhen HUANG Ru-zhu XIAO Xiang-xi WANG Zhi-jie |
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| Affiliation: | 1.Fujian Academy of Forestry , Fuzhou 350012 , Fujian, China;2.College of Life Sciences, Fujian Normal University , Fuzhou 350007, Fujian, China |
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| Abstract: | Six methods including basic CTAB method , modified CTAB methods as CTAB I , CTAB Ⅱ , CTAB Ⅲ , CTAB Ⅳ , and improved SDS methods were used to extract high-quality DNA from the young leaves of Betula luminifera .The DNA samples obtained by the above methods were tested by agarose-gel electrophoresis , restricted enzyme digests and PCR. Results showed that the modified methods were better than the basic CTAB method in terms of the quality of total DNA , but the effects of DNA extraction differed greatly . Among them, the modified CTAB I method was the best for extracting B .luminifera DNA from leaves .The comparisons of PCR patterns of genomic DNA extracted with different methods showed that different extracting methods would affect the changes of PCR patterns . Ch , 3 fig .1 tab .13 ref .] |
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