Prognostic significance of Ki67 evaluated by flow cytometry in dogs with high‐grade B‐cell lymphoma |
| |
| Authors: | A Poggi B Miniscalco E Morello F Gattino A Delaude L Ferrero Poschetto L Aresu M E Gelain V Martini S Comazzi F Riondato |
| |
| Affiliation: | 1. Department of Veterinary Sciences, University of Turin, Grugliasco, Italy;2. Department of Comparative Biomedicine and Food Science, University of Padua, Legnaro, Italy;3. Department of Veterinary Sciences and Public Health, University of Milan, Milan, Italy |
| |
| Abstract: | Ki67 can discriminate between high‐ and low‐grade canine lymphomas, but its prognostic role in specific subtypes of the neoplasm is unknown. We assessed the prognostic significance of Ki67% (percentage of Ki67‐positive cells), evaluated by flow cytometry, in 40 dogs with high‐grade B‐cell lymphoma, treated with a modified Wisconsin–Madison protocol (UW‐25). The following variables were investigated for association with lymphoma specific survival (LSS) and relapse free interval (RFI): Ki67%, breed, sex, age, stage, substage, complete remission (CR). By multivariate analysis, Ki67% (P = 0.009) and achievement of CR (P = 0.001) were independent prognostic factors for LSS. Dogs with intermediate Ki67% (20.1–40%) presented longer LSS and RFI (median = 866 and 428 days, respectively) than dogs with low (median = 42 days, P < 0.001; median = 159 days, P = 0.014) or high (median = 173 days, P = 0.038; median = 100 days, P = 0.126) values. Determination of Ki67 is a prognostic tool that improves the clinical usefulness of flow cytometric analysis in canine high‐grade B‐cell lymphoma. |
| |
| Keywords: | dog flow cytometry Ki67 lymphoma prognosis |
|
|
