| 甜瓜枯萎病拮抗菌的筛选及鉴定 |
| |
| 引用本文： | 余小兰,邹立飞,邹雨坤,李光义,李晓亮,李勤奋.甜瓜枯萎病拮抗菌的筛选及鉴定[J].南方农业学报,2018,49(6):1118-1124. |
| |
| 作者姓名： | 余小兰 邹立飞 邹雨坤 李光义 李晓亮 李勤奋 |
| |
| 作者单位： | 中国热带农业科学院 环境与植物保护研究所,海口 571101农业农村部儋州农业环境科学观测实验站,海南 儋州571737兴义民族师范学院,贵州 兴义 562400海南大学 环境与植物保护学院,海口 570228中国热带农业科学院 热带作物品种资源研究所,海南 儋州,571700 |
| |
| 摘 要： | 目的]筛选对甜瓜枯萎病菌有较强拮抗活性的菌株,并对拮抗菌株进行分类鉴定,为丰富热带地区甜瓜枯萎病生防菌资源库及田间应用提供参考.方法]以前期筛选获得的82株菌株为试验材料,通过平板对峙、继代培养、发芽率及盆栽试验筛选出对甜瓜枯萎病菌有较强拮抗活性的菌株,并通过形态特征、生理生化特征及16S rDNA序列分析对筛选获得的菌株进行分类鉴定.结果]筛选出一株对甜瓜枯萎病菌有较强拮抗活性的放线菌菌株D2,其对枯萎病的防治效果达73.53%.D2菌株可形成气生菌丝和孢子丝,孢子丝呈螺旋形,孢子卵呈圆形,且与利迪链霉菌(Streptomyces lydicus)聚于同一分支中,其同源性达99.6%;综合D2菌株的形态特征及生理生化特性,可鉴定其为利迪链霉菌( S. lydicus ).结论]D 2菌株对甜瓜枯萎病有较好的防治效果,具有开发成生物农药或生物肥料的潜力.
|
| 关 键 词： | 甜瓜枯萎病 拮抗菌 筛选 鉴定 热带地区 |
Screening and identification of antagonistic bacteria to muskmelon fusarium wilt |
| |
| YU Xiao-lan,ZOU Li-fei,ZOU Yu-kun,LI Guang-yi,LI Xiao-liang,LI Qin-fen.Screening and identification of antagonistic bacteria to muskmelon fusarium wilt[J].Journal of Southern Agriculture,2018,49(6):1118-1124. |
| |
| Authors: | YU Xiao-lan ZOU Li-fei ZOU Yu-kun LI Guang-yi LI Xiao-liang LI Qin-fen |
| |
| Abstract: | Objective]Antagonistic bacteria with strong antagonistic effect to fusarium wilt of melon was screened and identified to provide reference for enriching the resources of biocontrol bacteria against melon fusarium wilt in tropi-cal zone.Method]Eighty-two strains obtained in preliminary screening were used as experimental material,and antago-nistic bacteria against melon fusarium wilt were screened by using plate confront culture,successive transfer culture,ger-mination experiment and pot experiment. The strains were classified through characteristics in morphology,physiology and biochemistry and information of 16S rDNA.Result]A strain of actinomycetes D2 with strong antagonistic effect to melon fusarium wilt was screened and it had 73.53% control efficiency to fusarium wit of muskmelon. Strain D2 would form aerial hyphae and fibrillae of spores,and fibrillae of spores were spiral,and spore eggs were round. Strain D2 and Streptomyces lydicus were clustered in the same branch with 99.6% homology. Taking the morphological characters and physiological and biochemical characters of strain D2 into consideration,strain D2 was finally identified as S. lydicus.Conclusion]Strain D2 has strong antagonistic effect to fusarium wilt of melon and worth to be developed as biological pesticides and fertilizers. |
| |
| Keywords: | |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《南方农业学报》浏览原始摘要信息 |
|
点击此处可从《南方农业学报》下载全文 |
|
