| 土沉香幼苗对硝普钠—酸铝互作的生理响应 |
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| 引用本文： | 付晓凤,钟铭隆,朱原,王凌晖,黄玲璞.土沉香幼苗对硝普钠—酸铝互作的生理响应[J].南方农业学报,2018,49(4):676-682. |
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| 作者姓名： | 付晓凤 钟铭隆 朱原 王凌晖 黄玲璞 |
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| 作者单位： | 广西大学 林学院,南宁,530004广西国有博白林场,广西 玉林,537600四川农业大学 风景园林学院,成都,611130 |
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| 摘 要： | 目的]分析土沉香幼苗对硝普钠(SNP)—酸铝(AlCl3)互作的生理响应,为探讨土沉香的耐AlCl3胁迫机理提供理论依据.方法]采用完全随机设计,以不同浓度AlCl3胁迫土沉香幼苗并进行SNP-AlCl3互作处理,测定不同处理幼苗的光合色素和可溶性糖含量等生理指标,同时采用隶属函数模糊分析进行综合评价.结果]土沉香幼苗叶片的光合色素、可溶性糖和可溶性蛋白含量在0.2 mmol/L AlCl3胁迫下均出现最大值,在0.8 mmol/L AlCl3胁迫下均出现最小值;丙二醛(MDA)含量在0.2 mmol/L AlCl3胁迫下最低,在0.8 mmol/L AlCl3胁迫下最高,而超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性相反.添加SNP后,土沉香幼苗叶片的叶绿素a、叶绿素b、类胡萝卜素、可溶性糖和可溶性蛋白含量及SOD、POD和CAT活性等均明显高于未添加SNP处理.相关性分析结果表明,叶绿素总量、叶绿素a、叶绿素b、类胡萝卜素、可溶性糖和可溶性蛋白含量及SOD、POD和CAT活性等指标间均呈极显著正相关(P<0.01).结论]低浓度(0.2 mmol/L)AlCl3胁迫可促进土沉香幼苗生长,外源添加SNP对高浓度(0.8 mmol/L)AlCl3胁迫土沉香幼苗产生的毒害具有一定缓解作用,可在土沉香幼苗培育及抗性研究等相关领域推广应用.
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| 关 键 词： | 土沉香 酸铝(AlCl3)胁迫 硝普钠(SNP) 生理指标 |
Physiological responses of Aquilaria sinensis seedlings under SNP-AlCl3 interaction stress |
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| FU Xiao-feng,ZHONG Ming-long,ZHU Yuan,WANG Ling-hui,HUANG Ling-pu.Physiological responses of Aquilaria sinensis seedlings under SNP-AlCl3 interaction stress[J].Journal of Southern Agriculture,2018,49(4):676-682. |
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| Authors: | FU Xiao-feng ZHONG Ming-long ZHU Yuan WANG Ling-hui HUANG Ling-pu |
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| Abstract: | Objective]The physiological response of Aquilaria sinensis seedlings to sodium nitroprusside(SNP)-acid aluminum(AlCl3)interaction stress were analyzed to provide theoretical basis for further studying the resistance mecha-nism of A. sinensis against AlCl3stress.Method]A completely random design was applied,A. sinensis seedlings were stressed by different concentrations of AlCl3and treated with SNP-AlCl3interaction.Physiological indexes such as photo-synthetic pigment and soluble sugar content of A.sinensis under different treatment were detected.Fuzzy analysis method of membership function was used for comprehensive evaluation.Result]Photosynthetic pigments,soluble sugar content and soluble protein content in the seedling leaves of A.sinensis reached the maximum under 0.2 mmol/L AlCl3stress,and were the minimum under 0.8 mmol/L AlCl3stress. Malondialdehyde(MDA)content were the lowest when under 0.2 mmol/L AlCl3stress,and were the highest when under 0.8 mmol/L AlCl3stress,while activities of superoxide dismutase (SOD),peroxidase(POD)and catalase(CAT)were on the contrary.After adding SNP the physiological indexes in A.si-nensis seedling leaves such as contents of chlorophyll a,chlorophyll b,carotenoid,soluble sugar and soluble protein activi-ties of SOD,POD,CAT were largely higher than those without SNP.Correlation analysis indicated that,for the indexes in-cluding contents of total chlorophyll,chlorophyll a,chlorophyll b,carotenoid,soluble sugar and soluble protein,activities of SOD,POD and CAT there were extremely significant positive correlation between one and another(P<0.01).Conclu-sion]Applying low concentration(less than 0.2 mmol/L)AlCl3solution promotes A.sinensis seedlings growth.Exogenous addition of SNP has obvious mitigative effects on the toxicity caused by high concentration(0.8 mmol/L)AlCl3stress in A. sinensis seedlings,which can be applied in the related field such as cultivation and resistance research of A.sinensis seed-lings. |
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