| 样本数量对白桦群体遗传参数估算的影响 |
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| 引用本文： | 毕志宏,魏敏静,刘莹莹,杨传平,魏志刚.样本数量对白桦群体遗传参数估算的影响[J].浙江农林大学学报,2016,33(4):564-570. |
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| 作者姓名： | 毕志宏 魏敏静 刘莹莹 杨传平 魏志刚 |
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| 作者单位： | 东北林业大学 林木遗传育种国家重点实验室, 黑龙江 哈尔滨 150040 |
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| 基金项目： | 国家高技术研究发展计划("863"计划)项目2011AA100202 |
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| 摘 要： | 群体遗传参数是群体遗传学研究的主要内容之一,估算值的大小受群体样本数量的影响。以帽儿山试验林场白桦Betula platyphylla种源试验林为对象,采用序列相关扩增多态性(sequence-related amplified polymorphism,SRAP)技术分析了种源内不同样本数量对白桦群体遗传多样性的影响。结果表明:不同样本数量对白桦的估算的各遗传参数有一定影响,但影响各异。其中,对白桦群体多态位点百分率、群体内遗传变异和群体间遗传参数影响显著,对总位点数、多态位点数和基因流影响不显著。样本数量对于群体间与群体内遗传变异的大小的研究结果也产生影响,如样本数量超过8个,显示白桦种源内的遗传变异幅度大于种源间;而样本数量为4个,显示为种源间遗传变异幅度大于种源内。此外,不同样本数量揭示出的种源间亲缘关系不同,如样本数量超过8个,15个种源的聚类结果基本一致;而样本数量为4个,聚类结果无法反映白桦群体的亲缘关系。最后,提出了白桦遗传多样性研究时样本数量的计算公式,认为白桦多态位点百分率达98.5%的样本数量应为11个。
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| 关 键 词： | 林木遗传育种学 白桦 遗传多样性 样本容量 |
| 收稿时间： | 2015-06-10 |
Genetic parameters of Betula platyphylla provenances due to sample size |
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| BI Zhihong,WEI Minjing,LIU Yingying,YANG Chuanping,WEI Zhigang.Genetic parameters of Betula platyphylla provenances due to sample size[J].Journal of Zhejiang A&F University,2016,33(4):564-570. |
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| Authors: | BI Zhihong WEI Minjing LIU Yingying YANG Chuanping WEI Zhigang |
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| Affiliation: | State Key Laboratory of Forest Genetics and Breeding, Northeast Forestry University, Harbin 150040, Heilongjiang, China |
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| Abstract: | Population genetic parameters, one of the main constituents of population genetics, and the size of estimated values are influenced by the number of population samples. In this study Betula platyphylla (birch) from Cap Mountain Experimental Forest Farm's Provenance Testing Forest was chosen as the experimental object and sequence related amplified polymorphism(SRAP) technology was used for analysis. Results showed that genetic parameters with different sample sizes had different effects on birch. Genetic variation, population of birch groups, and the percentage of polymorphic numbers within populations influenced genetic parameters. Influence from genetic variation and genetic parameters between groups was strong, but total numbers, polymorphic numbers, and gene flow were not affected. When the sample size was greater than eight, genetic variation amplitude of the birch source was greater than that of the provenances; when the sample size was four, genetic variation was greater than the source of genetic variation among provenances for different samples. For genetic relationships of different provenances with sample size greater than eight, 15 kinds of source clustering results were basically the same. However, with a sample size of four, clustering results did not reflect the evolutionary relationship between birch groups. Also, 11 birch samples were found to have a polymorphic number that was 98.5%. |
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