Comparison of assays for the detection of West Nile virus antibodies in chicken serum |
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| Authors: | Hana M Weingartl Michael A Drebot Zden k Hublek Ji&#x;Í Halouzka Maya Andonova Antonia Dibernardo Colleen Cottam-Birt June Larence and Peter Marszal |
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| Affiliation: | Hana M. Weingartl, Michael A. Drebot, Zdeněk Hubálek, Ji?Í Halouzka, Maya Andonova, Antonia Dibernardo, Colleen Cottam-Birt, June Larence, and Peter Marszal |
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| Abstract: | Six tests for the detection of West Nile virus (WNV) antibodies in the serum of experimentally infected chickens were compared. The tests included the hemagglutination-inhibition test (HIT), immunoglobulin M (IgM)-capture enzyme-linked immunosorbent assay (ELISA) with WNV-infected mouse brain antigen, immunoglobulin G (IgG) indirect ELISA with tickborne encephalitis viral antigen, the microtitre virus neutralization test, the standard plaque reduction neutralization test (PRNT), and the microtitre PRNT (micro-PRNT). Thirty adult chickens, intravenously and intramuscularly inoculated with 107 plaque-forming units (PFU) of WNV strain Egypt 101, were bled and given a booster of 107 PFU at 7, 15, and 21 d postinoculation; the final blood collection was on day 28. Although the micro-PRNT is capable of detecting the highest antibody titres during both early and late infection, because of the technical complexity and time requirements of this test a combination of IgM and IgG ELISAs is recommended for serologic screening. Serum samples that give positive results in the ELISAs can then be tested by the micro-PRNT to determine the specificity of antibodies to WNV. |
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