| 应用RAPD 技术探讨红叶李、李和杏的亲缘关系 |
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| 引用本文： | 程晓建,杨萍,林伯年,郑炳松.应用RAPD 技术探讨红叶李、李和杏的亲缘关系[J].浙江农林大学学报,2004,21(1):40-43. |
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| 作者姓名： | 程晓建 杨萍 林伯年 郑炳松 |
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| 作者单位： | 1.浙江林学院 生命科学学院, 浙江 临安 311300;2.浙江大学 农业与生物技术学院, 浙江 杭州 310029 |
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| 基金项目： | 浙江省科学技术厅重大项目资助(021102537) |
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| 摘 要： | 为了对红叶李Prunus cerasifera cv .Atropurpurea , 李P .salicina 和杏P .armeniaca 的亲缘关系、品种识别及资源保存提供分子生物学依据, 也为了验证传统的形态学分类方法,从132 个随机引物中筛选出20 个引物对红叶李、李和杏的8 个材料进行了基因组DNA 扩增,均具有多态性, 共扩增出264 条带, 平均每个引物产生13.2 个RAPD 片段。RAPD 带型及聚类分析表明:RAPD 技术能将红叶李、李属植物和杏属植物完全分开, 红叶李、李和杏之间表现出一定的亲缘关系, 各属品种之间都有不同的遗传距离, 证明RAPD 技术可作为种和属水平的分类鉴定依据;利用红叶李、李和杏品种的特异谱带结合DNA 指纹, 可将参试的各品种鉴别出来, 从而说明了RAPD 技术能够用于种或品种之间的鉴定。图2 表2 参12
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| 关 键 词： | 植物学 红叶李 李 杏 随机扩增多态性DNA (RAPD) 亲缘关系 鉴定 |
| 收稿时间： | 2003-10-27 |
Genetic relationship of myrobalan plum,plum and apricot cultivars by RAPD analysis |
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| CHENG Xiao-jian,YANG Ping,LIN Bo-nian,ZHENG Bing-song.Genetic relationship of myrobalan plum,plum and apricot cultivars by RAPD analysis[J].Journal of Zhejiang A&F University,2004,21(1):40-43. |
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| Authors: | CHENG Xiao-jian YANG Ping LIN Bo-nian ZHENG Bing-song |
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| Affiliation: | 1..School of Life Sciences, Zhejiang Forestry College , Lin’an 311300, Zhejiang , China;2.College of Agriculture and Biotechnology , Zhejiang University , Hangzhou 310029, Zhejiang , China |
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| Abstract: | To provide evidence for conservation and identification of genetic relationship among myrobalan plum, plum and apricot cultivars and testify the traditional morphology-based taxonomy , genomic DNA's of 8 accessions are amplified by RAPD (random amplified polymorphic DNA)with 20 primers selected from 132 random primers .The result shows that they are all of polymorphism.264 fragments had been amplified , with 13.2 RAPD fragments amplified from each primer on the average .Clustering analysis shows that myrobalan plum , plum and apricot cultivars can be distinguished by RAPD ;there is some genetic relationship among them ;and the distance between varieties of each genus is different .The genetic distance reflects genetic relationship among tested materials .It has been proved that RAPD technique can be used for the classification and identification of genera .We can identify each cultivar of tested materials by DNA fingerprints and the specific bands .It shows that RAPD can be used for the identification of species or cultivars . Ch , 2 fig .2 tab .12 ref .] |
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