| 丁氟螨酯在蚯蚓体内的富集及其急性毒性 |
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| 引用本文： | 石琳琳,杨灿灿,陈琪,徐军,张平,何林.丁氟螨酯在蚯蚓体内的富集及其急性毒性[J].农药学学报,2022,24(1):177-181. |
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| 作者姓名： | 石琳琳 杨灿灿 陈琪 徐军 张平 何林 |
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| 作者单位： | 1.西南大学 植物保护学院，重庆 400715 |
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| 基金项目： | 国家自然科学基金 (31972297, 31801745) |
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| 摘 要： | 丁氟螨酯 (CYF) 对土壤中非靶标动物蚯蚓的影响尚未明确。本研究建立了蚯蚓体内丁氟螨酯的检测方法。样品经乙腈提取，采用液-液萃取法去除样品中的油脂，利用正相液相色谱 (NP-HPLC) 进行检测。结果表明:在0.5、5和10 mg/kg 3个添加水平下，CYF在蚯蚓中的回收率在84%~88%之间，相对标准偏差在1.3%~3.1%之间，验证了该提取方法的可靠性。生物-土壤富集因子 (BSAF) 测定结果表明，蚯蚓富集CYF 7 d后两个对映体((+)/(?)? CYF)在蚯蚓体内的富集浓度达到最大值，且 (+)-CYF的富集能力显著高于 (?)-CYF的。蚯蚓在含0.5~10 mg/kg CYF的土壤中暴露28 d时会严重抑制其体重。通过滤纸法检测CYF对蚯蚓的急性毒性，测得CYF消旋体对蚯蚓的LC50值为162.5 mg/L，(?)-CYF对蚯蚓的LC50值为323.1 mg/L，(+)-CYF对蚯蚓的LC50值为309.4 mg/L。根据联合毒性效应分析得到AI = ?0.03，表明同时使用CYF的两个对映体对蚯蚓造成毒性相加作用。
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| 关 键 词： | 丁氟螨酯 杀螨剂 蚯蚓 生物-土壤富集因子 体重 急性毒性 |
| 收稿时间： | 2021-06-08 |
Accumulation and acute toxicity of cyflumetofen in earthworms |
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| SHI Linlin,YANG Cancan,CHEN Qi,XU Jun,ZHANG Ping,HE Lin.Accumulation and acute toxicity of cyflumetofen in earthworms[J].Chinese Journal of Pesticide Science,2022,24(1):177-181. |
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| Authors: | SHI Linlin YANG Cancan CHEN Qi XU Jun ZHANG Ping HE Lin |
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| Affiliation: | 1.College of Plant Protection, Southwest University, Chongqing 400715, China2.Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China |
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| Abstract: | The effect of cyflumetofen (CYF) on non-target organism earthworms (Eisenia fetida) in soil is unclear. In this study, a method was established for detection of CYF in earthworms. The samples were extracted with acetonitrile, the lipid in the earthworms was removed by liquid-liquid extraction, and then the concentration of CYF was detected by normal phase-HPLC. The results showed that the recoveries were 84%-88%, and the relative standard deviation was 1.3%-3.1% at the spiking levels of 0.5, 5, and 10 mg/kg, which verified the reliability of the method. According to the Biota-soil accumulation factor (BSAF), the enrichment concentration of the two enantiomers reached the maximum on the 7th day that the earthworms were enriched with CYF, and the enrichment ability of (+)-CYF in the earthworms was significantly higher than that of (?)-CYF. Long-term exposure of 0.5-10 mg/kg CYF for 28 days seriously inhibited the body weight of earthworms. The acute toxicity of cyflumetofen to earthworms was detected by filter paper method. The LC50 of rac-CYF, (?)-CYF, and (+)-CYF to earthworms was 162.5, 323.1 and 309.4 mg/L, respectively. According to the combined toxicity analysis, AI = ?0.03 was obtained. The simultaneously use of the two enantiomers of CYF will cause additive toxicity to earthworms. |
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