| 装载鸡传染性支气管炎病毒 S1蛋白重组HD11细胞源外体的构建 |
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| 引用本文： | 谢言,邢薿文,肖倩,覃尧.装载鸡传染性支气管炎病毒 S1蛋白重组HD11细胞源外体的构建[J].热带生物学报,2022,13(6):562-568. |
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| 作者姓名： | 谢言 邢薿文 肖倩 覃尧 |
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| 作者单位： | 海南大学 动物科技学院， 海口 570228 |
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| 基金项目： | 国家自然科学基金项目（31802173）海南大学E类引进人才科研启动项目[KYQD(ZR)1937]；海南省重点研发计划项目（社会发展）[ZDYF2020144] |
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| 摘 要： | 为了给禽类巨噬细胞源外体在免疫应答的功能研究和家禽外体载体疫苗的研发奠定基础，本研究以鸡传染性支气管炎病毒(Infectious bronchins virus, IBV)S1蛋白作为模式抗原，通过慢病毒表达系统技术实现在HD11细胞中稳定表达S1蛋白，再利用超速离心从细胞培养物上清中分离纯化得到外体。蛋白免疫印分析、电镜与粒径分析检测等结果表明，在该重组HD11细胞分泌的外体中可检测到稳定的S1蛋白表达，显示成功构建了装载IBV S1蛋白的重组外体。
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| 关 键 词： | 外体 HD11细胞 IBV S1蛋白 |
| 收稿时间： | 2022-02-06 |
Construction of exosomes of recombinant HD 11 cells harboring the IBV S1 protein |
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| Affiliation: | School of Animal Science and Technology, Hainan University, Haikou, Hainan 570228, China |
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| Abstract: | The exosomes of antigen presenting cells (Antigen presenting cell, APC) participate in the immune response process, and can activate the adaptive immune response after carrying the antigen. As a kind of antigen-presenting cells, the related functions of poultry macrophage-derived exosomes are still unknown. The S1 protein of chicken infectious bronchitis virus (IBV) was used as the model antigen, the S1 protein was stably expressed in HD11 cells through the lentiviral expression system, and then the exosomes were separated and purified from the cell culture supernatant by ultracentrifugation. The protein immunoblot analysis, electron microscopy and particle size analysis detection showed that stable S1 protein expression can be detected in the exosomes secreted by the recombinant HD11 cells. Recombinant exosomes harboring IBV S1 protein were successfully constructed, which can provide a reference for the functional study of avian macrophage-derived exosomes in immune response and the development of poultry exosome vector vaccines. |
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