Sperm characteristics of chub Leuciscus cephalus (L.) collected in artificial condition after Ovopel and Ovaprim treatment |
| |
| Authors: | Beata Irena Cejko S?awomir Krejszeff |
| |
| Affiliation: | 1. Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland;2. Department of Lake and River Fisheries, Faculty of Environmental Sciences, University of Warmia and Mazury, Olsztyn, Poland |
| |
| Abstract: | The effect of two commercial preparations containing different GnRH analogues with dopamine antagonists on quantitative and qualitative parameters of semen from chub Leuciscus cephalus L. collected in artificial conditions were examined. Semen was collected after the application of (D‐Ala6, Pro9 NEt)‐mGnRH + metoclopramide] (Ovopel, n = 9), (D‐Arg6, Pro9 Net)‐sGnR + domperidone] (Ovaprim, n = 9) and from the control group (0.9% NaCl, n = 9). Afterwards, semen volume, sperm concentration, total sperm production and semen pH were determined. Osmolality and pH of seminal plasma were also determined. Using the Computer‐assisted sperm analysis system (CASA), selected sperm parameters such as sperm motility (MOT %), progressively motile sperm (PRG, %), curvilinear velocity (VCL, μm s?1), straight‐line velocity (VSL, μm s?1), movement linearity (LIN, %), wobbling index (WOB, %), amplitude of lateral head displacement (ALH, μm) and beat cross frequency (BCF, Hz) were analysed. While Ovopel can also be used to stimulate chub spermation, the application of Ovaprim was much more effective for obtaining higher amounts of semen. |
| |
| Keywords: | chub sperm seminal plasma
CASA
Ovopel Ovaprim |
|
|
