| 山西某鸡棚改造育肥猪场腹泻病因诊断 |
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| 引用本文： | 郑龙龙,潘国梅,张黎,谈小龙.山西某鸡棚改造育肥猪场腹泻病因诊断[J].中国猪业,2021,16(3):62-64. |
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| 作者姓名： | 郑龙龙 潘国梅 张黎 谈小龙 |
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| 作者单位： | 1山西农业大学动物医学学院,山西太谷 030801;2云南省楚雄州禄丰县动物卫生监督所,云南禄丰 652100;3安徽省六安市畜牧发展中心,安徽六安 237000 |
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| 基金项目： | 山西省科技成果转化项目“微生态制剂养殖场就地生产示范基地建设”（SXNKTG201802） |
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| 摘 要： | 为诊断山西省某鸡棚改猪场80~100日龄猪群发生腹泻病的病因。采集40份猪的肛拭子样品,通过实时荧光扩增和细菌分离鉴定的方法,进行实验室诊断。检测结果显示,腹泻样品中轮状病毒（Porcine rotavirus, PoRV）核酸100%为阳性,猪圆环病毒2型（Porcine circovirus type 2, PCV-2）核酸30%为阳性。猪流行性腹泻病毒(Porcine epidemic diarrhea, PED)、猪伪狂犬野毒(Pseudorabies virus, PrV)、猪瘟病毒(Swine fever virus, SFV)核酸检测均为阴性,大肠杆菌（Escherichia coli, E.coli）和沙门氏菌(Salmonella enteriditis, SE)100%呈阳性,仅对万古霉素和头孢曲松同时敏感。
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| 关 键 词： | 轮状病毒 猪圆环病毒2型 大肠杆菌 沙门氏菌 |
| 收稿时间： | 2021-02-26 |
Etiological Diagnosis of Diarrhea in a Fattening Pig Farm of Shanxi Province |
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| ZHENG Longlong,PAN Guomei,ZHANG Li,TAN Xiaolong.Etiological Diagnosis of Diarrhea in a Fattening Pig Farm of Shanxi Province[J].China Swine Industry,2021,16(3):62-64. |
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| Authors: | ZHENG Longlong PAN Guomei ZHANG Li TAN Xiaolong |
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| Affiliation: | 1College of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China;2Lufeng Animal Health Supervision, Chuxiong 652100, China;3Lu'an Animal Husbandry Development Center, Lu'an 237000, China |
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| Abstract: | In order to analyze of the cause of diarrhea in 80 to 100-day-old pigs in a poultry farm in Shanxi Province, 40 samples of fresh diarrhea swab through Q-PCR and bacteria separation and identification methods for laboratory diagnosis, PoRV and PCV-2 positive for the virus nucleic acid, pathogenic E.coli and salmonella was positive. The experimental results showed that 40 samples of diarrhea PoRV nucleic acids were all positive, PCV-2 nucleic acid 30% were positive, PED, Prv, SFV nucleic acid were negative, E.coli and SE were all positive. |
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| Keywords: | PoRV PCV-2 E coli SE |
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