| 除草活性菌株HZ-011发酵培养基筛选及其条件优化 |
| |
| 引用本文： | 蔺泽荣,朱海霞.除草活性菌株HZ-011发酵培养基筛选及其条件优化[J].南方农业学报,2021,52(7):1931-1941. |
| |
| 作者姓名： | 蔺泽荣 朱海霞 |
| |
| 作者单位： | 1 青海大学, 西宁 810016;2 青海省农林科学院, 西宁 810016;3 农业农村部西宁作物有害生物科学观测实验站, 西宁 810016;4 青海省农业有害生物综合治理重点实验室, 西宁 810016 |
| |
| 基金项目： | 青海省应用基础研究计划项目（2019-ZJ-7057） |
| |
| 摘 要： | 【目的】明确除草活性菌株HZ-011的发酵培养基各因素配比及最适发酵条件，为该菌作为除草剂的研发和农田应用提供理论依据。【方法】以产孢量为目标，通过固体培养基筛选确定菌株HZ-011生长的基础培养基；采用单因素试验优化菌株HZ-011的发酵培养基和最佳发酵条件；利用中心组合设计（Central composite design，CCD）原理对菌株HZ-011的液体培养基成分及配比进行优化；通过响应面法分析培养基各成分间的交互作用；用菌株HZ-011发酵液对盆栽杂草（藜、密花香薷、冬葵和反枝苋）进行致病性试验。【结果】经过优化，菌株HZ-011的培养基最佳配比为蔗糖48.744 g/L、蛋白胨15.626 g/L、NaCl 0.214 g/L和K2HPO4 0.428 g/L，最佳发酵条件为pH 7、温度25℃、培养时间5 d、装液量180 mL和转速180 r/min。盆栽试验结果表明，菌株HZ-011对藜和反枝苋全部致死，对密花香薷的致病率为75%，对冬葵的致病率为40%。【结论】优化后的培养基提高了菌株HZ-011的产孢量，为该菌株应用于农田杂草生物防治打下基础。
|
| 关 键 词： | 菌株HZ-011 除草活性 发酵条件 单因素 产孢量 响应面 |
| 收稿时间： | 2021-03-31 |
Screening of fermentation medium for herbicidal active strain HZ-011 and optimization of its conditions |
| |
| LIN Ze-rong,ZHU Hai-xia.Screening of fermentation medium for herbicidal active strain HZ-011 and optimization of its conditions[J].Journal of Southern Agriculture,2021,52(7):1931-1941. |
| |
| Authors: | LIN Ze-rong ZHU Hai-xia |
| |
| Affiliation: | 1 Qinghai University, Xining, 810016, China;2 Qinghai Academy of Agriculture and Forestry Sciences, Xining 810016, China;3 Xining Crop Pest Scientific Observation and Experiment Station of the Ministry of Agriculture and Rural Affairs, Xining 810016, China;4 Qinghai Provincial Key Laboratory of Comprehensive Management of Agricultural Pests, Xining 810016, China |
| |
| Abstract: | 【Objective】 Todetermine the ratio of various factors of the fermentation medium and the optimal fermentation conditions of strain HZ-011 that has herbicidal effect on weeds, andprovide theoretical basis for development of HZ-011 herbicide and application in farmland.【Method】 The basic medium for the growth of strain HZ-011 was selected by solid medium with the goal of spore production. Single factor experiment was used to optimize the fermentation medium and fermentation conditions of strain HZ-011. The liquid medium composition and ratio of strain HZ-011 were optimized by using the principle of central composite design(CCD). The interaction between the components of the medium was analyzed by response surface methodology. The pathogenicity of potted weeds(Chenopodium album, Amaranthus retroflexus, Elsholtzia densa and Malva crispa) was tested with HZ-011 fermentation broth.【Result】 After optimization, the optimal proportion of culture medium of strain HZ-011 was sucrose 48.744 g/L, peptone 15.626 g/L, NaCl 0.214 g/L and K2 HPO40.428 g/L. The optimum fermentation conditions were p H 7, temperature 25 ℃, culture time 5 days, liquid loading 180 ml and rotating speed 180 r/min. The results of pot experiment showed that strain HZ-011 killed all C. album and A. retroflexus, and the pathogenicity of E. densa was 75%, then the pathogenic rate of winter sunflower was 40%.【Conclusion】 The optimized medium increased the spore production of strain HZ-011, which lays a foundation for the application of the strain in the biological control of farmland weeds. |
| |
| Keywords: | |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《南方农业学报》浏览原始摘要信息 |
|
点击此处可从《南方农业学报》下载全文 |
|
