| 植物激素和光暗培养条件对卷丹百合再生体系的影响 |
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| 引用本文： | 刘彦珍,郜新强,张元臣,陈思,郝静,吕有军.植物激素和光暗培养条件对卷丹百合再生体系的影响[J].南方农业学报,2021,52(5):1300-1309. |
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| 作者姓名： | 刘彦珍 郜新强 张元臣 陈思 郝静 吕有军 |
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| 作者单位： | 1. 安阳工学院生物与食品工程学院, 河南安阳 455000;2. 信阳涉外职业技术学院, 河南信阳 465550;3. 安阳市道路绿化管理站, 河南安阳 455000 |
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| 基金项目： | 河南省科技攻关计划项目（182102110209）；安阳市科技攻关项目（2018-124） |
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| 摘 要： | 【目的】探究植物激素和光暗培养条件对卷丹百合（Lilium lancifolium Thunb）再生体系的影响，为卷丹百合种质资源开发和大面积推广种植打下基础。【方法】以卷丹百合鳞片为外植体，分析培养基中不同植物激素［NAA、6-BA、2，4-D、噻苯隆（TDZ）和毒莠定（PIC）］配比及光（光照时间14 h/d）、暗（全天黑暗）培养条件对卷丹百合鳞片愈伤组织诱导、增殖及不定芽再生的影响，建立卷丹百合组培高效再生体系。【结果】与不添加任何激素的对照（CK）相比，添加激素的培养基均能显著提高卷丹百合鳞片愈伤组织的诱导率（P<0.05），其中TDZ 0.4 mg/L+NAA 0.2 mg/L组合的效果最佳，暗培养条件下其愈伤组织诱导率达65.57%；在TDZ 0.4 mg/L+2，4-D 0.05 mg/L的培养基中暗培养20 d后，再转入TDZ 0.4 mg/L+NAA 0.2 mg/L的培养基中继续暗培养40 d，愈伤组织增殖倍数最高，可达2.45倍。6-BA与NAA组合比6-BA与PIC组合更利于诱导不定芽的产生，芽再生倍数最高的培养基为6-BA 0.6 mg/L+NAA 0.1mg/L，暗培养条件下其再生倍数最高可达6.08倍。与光培养相比，暗培养更利于愈伤组织诱导和增殖，但在愈伤组织诱导不定芽阶段，长时间暗培养不利于芽的生长，暗培养20 d后转移至光培养条件下继续培养40 d，生成的不定芽色泽鲜绿，叶片伸展，长势旺盛，利于壮苗和生根。【结论】卷丹百合组织培养的不同阶段受不同种类和浓度的植物激素组合影响；暗培养条件更利于愈伤组织的诱导和增殖，暗培养结合光培养条件最适合不定芽的再生和分化。
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| 关 键 词： | 卷丹百合 植物激素 光暗条件 再生体系 |
| 收稿时间： | 2021-02-04 |
Effects of plant hormones and light or dark culture conditions on the regeneration system of Lilium lancifolium Thunb |
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| LIU Yan-zhen,GAO Xin-qiang,ZHANG Yuan-chen,CHEN Si,HAO Jing,LYU You-jun.Effects of plant hormones and light or dark culture conditions on the regeneration system of Lilium lancifolium Thunb[J].Journal of Southern Agriculture,2021,52(5):1300-1309. |
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| Authors: | LIU Yan-zhen GAO Xin-qiang ZHANG Yuan-chen CHEN Si HAO Jing LYU You-jun |
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| Affiliation: | 1. College of Biological and Food Engineering, Anyang Institute of Technology, Anyang, Henan 455000, China;2. Xinyang International Vocation Institute, Xinyang, Henan 465550, China;3. Anyang City Road Greening Management Station, Anyang, Henan 455000, China |
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| Abstract: | 【Objective】In order to lay a foundation for germplasm resources development and large area popularization of Lilium lancifolium Thunb, investigated the effects of plant hormones and light and dark culture conditions on the regeneration system of lily.【Method】Using lily scales as explants, analyzing the effects on callus induction, proliferation and adventitious bud regeneration in lily scales affected by the ratio of different plant hormonesNAA, 6-BA, 2, 4-D, thidiazuron(TDZ) and 4-amino-3, 5, 6-trichloropicolinic acid(PIC)]in the medium and light(illumination time:14 h/d) and dark(dark all day) culture conditions, to establish an efficient regeneration system for lily tissue culture.【Result】Compared with the control that adding no hormones (CK), mediea with different hormone combinations could significantly improve the callus induction rate of L. lancifolium (P<0.05). The combination of TDZ 0.4 mg/L+NAA 0.2 mg/Lshowed the best induction effectiveness, and the callus induction rate reached 65.57% under dark culture conditions. The scales of L. lancifolium was firstly cultured in TDZ 0.4 mg/L+2, 4-D 0.05 mg/Lfor 20 d in the dark, and then cultured in TDZ 0.4 mg/L+NAA 0.2 mg/L for 40 d in dark condition. In this case, the proliferation rate of callus was the highest, up to 2.45 times. The combination of 6-BA and NAA was more favorable to the induction of adventitious buds than the combination of 6-BA and PIC. The medium with the highest regeneration multiple was 6-BA 0.6 mg/L+NAA 0.1 mg/L, and its regeneration multiple was up to 6.08 times in dark condition. Compared with light culture, the dark culture was more conducive to callus induction and proliferation, but in the adventitious bud stage of the callus, the long-time dark culture was adverse to the growth of the bud. After incubating for 20 d in dark condition, the scales of L. lancifolium were transferred to the light condition and continue to cultivating for 40 d, the generated adventitious buds were bright green in colour, and its leaves were spreading and growing vigorously. Such adventitious buds were helpful to strong seedling and rooting.【Conclusion】Different stages of tissue culture of L. lancifolium are affected by plant. Combination of different kinds and concentrations. Dark culture is more favorable to callus induction and proliferation, the combination of dark culture and light culture is the most suitable for the regeneration and differentiation of adventitious buds. |
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