| 栀子苷对双酚A致鲤鱼肝毒性的保护作用 |
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| 引用本文： | 顾郑琰,贾睿,何勤,曹丽萍,杜金梁,徐跑,殷国俊.栀子苷对双酚A致鲤鱼肝毒性的保护作用[J].南方农业学报,2021,52(2):501-508. |
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| 作者姓名： | 顾郑琰 贾睿 何勤 曹丽萍 杜金梁 徐跑 殷国俊 |
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| 作者单位： | 1. 南京农业大学无锡渔业学院, 江苏无锡 214081;2. 中国水产科学研究院淡水渔业研究中心/农业农村部淡水渔业和种质资源利用重点实验室, 江苏无锡 214081 |
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| 基金项目： | 国家自然科学基金项目（31702318）；中国水产科学研究院淡水渔业研究中心基本科研业务费专项（2021JBFM09） |
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| 摘 要： | 【目的】探究不同浓度栀子苷对双酚A（BPA）致鲤鱼（Cyprinus carpio）肝毒性的影响，为鱼类保肝药物的筛选提供依据。【方法】以75条鲤鱼为试验材料，设5个处理组，即空白对照组、模型组（0.5 mg/L BPA暴露）及栀子苷处理组（BPA+栀子苷），其中，栀子苷设低（1.25 g/kg）、中（2.50 g/kg）、高（5.00 g/kg）3个浓度，养殖70 d后，检测血清中谷丙转氨酶（GPT）、谷草转氨酶（GOT）、碱性磷酸酶（AKP）、甘油三酯（TG）、总胆固醇（TC）、高密度脂蛋白胆固醇（HDLC）和低密度脂蛋白胆固醇（LDL-C）的水平，肝组织中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽（GSH）、丙二醛（MDA）和总抗氧化能力（T-AOC）的水平，以及肝脏中fatp1、fas、ppar-α、nrf2、keap1、ho-1、tlr1、myd88、il-1β、il-6和il-10基因的表达量，来评价栀子苷对鲤鱼肝毒性的治疗效果。【结果】与对照组相比，模型组鲤鱼的特定生长率显著降低，饵料系数显著增高（P< 0.05，下同），而联合投喂3种浓度的栀子苷可显著提高特定生长率，降低饵料系数，改善鲤鱼的生长情况。与模型组相比，饲喂中、高浓度的栀子苷显著降低鲤鱼血清中GPT、GOT和AKP活性，缓解鲤鱼肝损伤的程度。饲喂低、中浓度的栀子苷显著降低血清中TG和LDL-C的水平和肝组织中fatp1和ppar-α基因的表达量，增加血清中HDL-C的含量，改善了鲤鱼肝脏脂质代谢紊乱。同时，经过3种浓度的栀子苷处理后，肝组织中SOD活性和nrf2、ho-1基因的表达量显著升高，keap1基因的表达量显著降低，表明栀子苷对双酚A引起的肝脏氧化应激有缓解作用。此外，饲喂3种浓度的栀子苷较模型组显著下调了myd88、il-1β和il-6基因的表达量，但显著上调了il-10基因的表达，抑制了鲤鱼肝脏的炎症反应。【结论】栀子苷可不同程度缓解双酚A引起的鲤鱼肝毒性，并通过改善肝脏脂质异常代谢，增强抗氧化能力，抑制炎症反应等，减轻鲤鱼肝损伤程度。考虑到实际生产成本，推荐以中浓度（2.50 g/kg）的栀子苷作为保肝药物的筛选依据。
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| 关 键 词： | 栀子苷 双酚A 保肝作用 肝毒性 鲤鱼 |
| 收稿时间： | 2020-10-13 |
Protective effects of geniposide on hepatotoxicity induced by bisphenol A in common carp(Cyprinus carpio) |
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| GU Zheng-yan,JIA Rui,HE Qin,CAO Li-ping,DU Jin-liang,XU Pao,YIN Guo-jun.Protective effects of geniposide on hepatotoxicity induced by bisphenol A in common carp(Cyprinus carpio)[J].Journal of Southern Agriculture,2021,52(2):501-508. |
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| Authors: | GU Zheng-yan JIA Rui HE Qin CAO Li-ping DU Jin-liang XU Pao YIN Guo-jun |
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| Affiliation: | 1. College of Wuxi Fisheries, Nanjing Agricultural University, Wuxi, Jiangsu 214081, China;2. Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences/Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Wuxi, Jiangsu 214081, China |
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| Abstract: | 【Objective】Explored the effects of different concentrations of geniposide on the hepatotoxicity of bisphenol A(BPA) in common carp(Cyprinus carpio), and provided a reference for screening hepatoprotective drug in fish.【Method】In this study, 75 common carps were divided into five treatment groups:blank control group, model group(0.5 mg/L BPA exposure) and geniposide treatment group(BPA+geniposide).Geniposide was set at low(1.25 g/kg), medium(2.50 g/kg) and high(5.00 g/kg) concentrations for 70 d.Then detected glutamic-pyruvic transaminase(GPT), glutamic oxalacetic transaminase(GOT), alkaline phosphatase(AKP), triglycerides(TG), total cholesterol(TC), high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein cholesterol(LDL-C) levels in the serum, super oxide dismutase(SOD), catalase(CAT), glutathione(GSH), malondialdehyde(MDA) and total antioxidant capacity(T-AOC) levels, as well as the expression levels of fatp1, fas, ppar-α, nrf2, keap1, ho-1, tlr1, myd88, il-1β, il-6 and il-10 genes in the liver to evaluate the therapeutic effects of geniposide on hepatotoxicity in common carp.【Result】Compared with the control group, the specific growth rate of common carp in the model group was significantly reduced, and the feed coefficient was significantly increased(P< 0.05, the same below).The combined feeding of three concentrations of geniposide could significantly increase the specific growth rate, reduce the feedcoefficient, and improve the growth of carp.Compared with model group, geniposide at middleandhighconcentrations significantly reduced the activities of GPT, GOT and AKP in common carp serum, and alleviated the degree of liver injury in common carp.Lowor middle concentrations of geniposide significantly reduced the levels of TG and LDL-C in serum and the gene expressions of fatp1 and ppar-αin liver tissue, and increased the content of HDL-C in serum, which improved common carp liver lipid metabolism disorder.At the same time, after three concentrations of geniposide treatment, the activity of SOD and the gene expressions of nrf2 and ho-1 in liver tissues were significantly increased, and the expression levels of keap1 gene was significantly decreased, indicating that geniposide could relieve liver oxidative stress caused by BPA.In addition, compared with model group, feeding three concentrations of geniposide significantly down-regulated the gene expressions of myd88, il-1βand il-6, significantly up-regulated the gene expression of il-10, which inhibited the inflammatory response in liver of common carp.【Conclusion】In summary, geniposide can alleviate common carp hepatotoxicity caused by BPA to varying degrees, and reduce hepatotoxicity by improving liver lipid metabolism, enhancing antioxidant capacity, and inhibiting inflammation.Taking into account the actual production cost, it is recommended to use geniposide at middle concentration(2.50 g/kg) as the basis for screening hepatoprotective drugs. |
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