| 草地贪夜蛾和斜纹夜蛾幼虫体内保护酶及解毒酶对2种杀虫剂的响应比较 |
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| 引用本文： | 李浩,陈亚平,鲁智慧,郭建洋,李亚红,朱林云,和淑琪,桂富荣.草地贪夜蛾和斜纹夜蛾幼虫体内保护酶及解毒酶对2种杀虫剂的响应比较[J].南方农业学报,2021,52(3):559-569. |
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| 作者姓名： | 李浩 陈亚平 鲁智慧 郭建洋 李亚红 朱林云 和淑琪 桂富荣 |
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| 作者单位： | 1. 云南农业大学植物保护学院/云南生物资源保护与利用国家重点实验室, 昆明 650201;2. 中国农业科学院植物保护研究所, 北京 100193;3. 云南省植保植检站, 昆明 650034;4. 云南省高原特色农业产业研究院, 昆明 650201 |
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| 基金项目： | 国家重点研发计划项目（2019YFD0300101，2019YFD0300104）；云南省重大科技专项（2019ZG00910） |
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| 摘 要： | 【目的】明确草地贪夜蛾Spodoptera frugiperda（J. E. Smith）]及其近缘种斜纹夜蛾Spodoptera litura（Fabricius）]幼虫体内保护酶和解毒酶对2种不同作用类型杀虫剂的响应，为草地贪夜蛾和斜纹夜蛾的田间防控及抗药性治理提供科学依据。【方法】采用叶片浸渍法测定甲维盐和氯虫苯甲酰胺对草地贪夜蛾和斜纹夜蛾3龄幼虫的毒力，并用2种杀虫剂亚致死浓度（LC25）和致死中浓度（LC50）处理过的小麦叶片分别饲喂2种害虫的3龄幼虫，比较取食6、12、18、24和48 h后幼虫体内保护酶超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）]及解毒酶谷胱甘肽S-转移酶（GST）、细胞色素P450（CYP450）和羧酸酯酶（CarE）]的活性变化。【结果】甲维盐对草地贪夜蛾和斜纹夜蛾的毒力高于氯虫苯甲酰胺。2类杀虫剂胁迫下2种害虫的CYP450活性均上升，且草地贪夜蛾的CYP450活性高于斜纹夜蛾，在甲维盐和氯虫苯甲酰胺低剂量（LC25）胁迫下草地贪夜蛾的CYP450活性均高于高剂量（LC50）胁迫处理，处理后48 h均达最高值（15.72±0.41和15.36±0.47 ng/mL）；而草地贪夜蛾另2种解毒酶（GST和CarE）活性在甲维盐处理后低于斜纹夜蛾。甲维盐胁迫下2种害虫体内保护酶（SOD和POD）活性均降低，且草地贪夜蛾保护酶活性低于斜纹夜蛾；氯虫苯甲酰胺胁迫下草地贪夜蛾保护酶（SOD和POD）活性均上升，高剂量氯虫苯甲酰胺胁迫下2种害虫SOD和CAT活性均高于低剂量胁迫处理，且草地贪夜蛾SOD和CAT活性高于斜纹夜蛾。2种害虫体内保护酶和解毒酶活性对2类杀虫剂响应差异明显，且物种、药剂、浓度、时间、物种×浓度和药剂×浓度因素对2种害虫保护酶和解毒酶活性均有极显著影响（P<0.01）。2类杀虫剂胁迫下2种害虫的SOD和CAT活性间相关性较强。【结论】草地贪夜蛾对甲维盐的敏感性高于斜纹夜蛾，而对氯虫苯甲酰胺的敏感性低于斜纹夜蛾，且2种害虫均主要通过增强CYP450活性对杀虫剂进行解毒代谢。农业生产上推荐使用甲维盐作为防治草地贪夜蛾的药剂。
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| 关 键 词： | 草地贪夜蛾 斜纹夜蛾 保护酶 解毒酶 抗药性 |
| 收稿时间： | 2021-03-04 |
Response comparison of protective and detoxification enzymes in Spodoptera frugiperda(J. E. Smith) and Spodoptera litura(Fabricius) larvae to two insecticides |
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| LI Hao,CHEN Ya-ping,LU Zhi-hui,GUO Jian-yang,LI Ya-hong,ZHU Lin-yun,HE Shu-qi,GUI Fu-rong.Response comparison of protective and detoxification enzymes in Spodoptera frugiperda(J. E. Smith) and Spodoptera litura(Fabricius) larvae to two insecticides[J].Journal of Southern Agriculture,2021,52(3):559-569. |
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| Authors: | LI Hao CHEN Ya-ping LU Zhi-hui GUO Jian-yang LI Ya-hong ZHU Lin-yun HE Shu-qi GUI Fu-rong |
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| Affiliation: | 1. Plant Protection College, Yunnan Agricultural University/State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan, Kunming 650201, China;2. Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;3. Yunnan Plant Protection and Quarantine Station, Kunming 650034, China;4. Yunnan Plateau Characteristic Agriculture Industry Research Institute, Kunming 650201, China |
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| Abstract: | 【Objective】The research aimed to clarify the response of different insecticides on the activities of protective enzymes and detoxification enzymes in the larvae of Spodoptera frugiperda(J. E. Smith) and Spodoptera litura(Fabricius) to two insecticides, and to provide theoretical basis for further study on the field control and and insecticide resistance mechanism of S. frugiperda and S. litura.【Method】The toxicity of emamectin and chlorantraniliprole to the 3rd instar larvae of S. frugiperda and S. litura were assayed by leaf-dipping method by feeding wheat leaves treated with sublethal concentration(LC25) and median lethal concentration(LC50) of the pesticides respectively, and the activities of protective enzymessuperoxide dismutase(SOD), peroxidase(POD) and catalase(CAT)]and detoxification enzymesglutathione S-transferase(GST), cytochrome P450(CYP450) and carboxylesterase(CarE)]in larvae after being treated for 6, 12, 18, 24 and 48 h were measured and analyzed.【Result】The toxicity of emamectin to S. frugiperda and S. litura was higher than that of chlorantraniliprole. The activity of CYP450 in both pests increased after the two insecticide treatments, and was significantly higher in S. frugiperda than that in S. litura. The CYP450 activity in S. frugiperda was more active at low concentration(LC25) of emamectin and chlorantraniliprole than at high concentration(LC50), which was the highest at 48 h as 15.72±0.41(emamectin) and 15.36±0.47 ng/mL(cholrantraniliprole). However, the activities of two detoxification enzymes GST and CarE in S. frugiperda were lower than those in S. litura after emamectin treatment. The activities of protective enzymes SOD and POD in both pests decreased after treated with emamectin, and were lower in S. frugiperda than those in S. litura;while the activities of SOD and POD in S. frugiperda increased after treated with chlorantraniliprole. The activities of SOD and CAT were higher in both pests at LC50 chlorantraniliprole than at LC25, and were higher in S. frugiperda than in S. litura. The response activities of protective enzymes and detoxification enzymes in both pests were greatly affected by the two insecticides, and species, insecticide, concentration, time, species×concentration and insecticide×concentration had extremely significant effects on the activities of protective enzymes and detoxification enzymes(P<0.01). There was a strong correlation between the activities of SOD and CAT of both S. frugiperda and S. litura under two insecticides treatments.【Conclusion】The susceptibility of S. frugiperda to emamectinis higher than that of S. litura, while the sensitivity to chlorantraniliprole is lower than that of S. litura, and the two pests enhance detoxification metabolism of insecticides by increasing CYP450 activity. In agricultural production, it is recommended to use emamectin as an agent for controlling S. frugiperda. |
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