| 益气解毒方对鼻咽癌CNE2细胞增殖的影响 |
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| 作者姓名： | 胡晶 刘洁 徐冰雁 范婧莹 罗晶婧 胡梅 何迎春 |
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| 作者单位： | 湖南中医药大学, 湖南 长沙 410208;长沙医学院中医学院, 湖南长沙 410219,湖南中医药大学, 湖南 长沙 410208,湖南中医药大学, 湖南 长沙 410208,湖南中医药大学, 湖南 长沙 410208;中医药防治眼耳鼻咽喉疾病湖南省重点实验室, 湖南 长沙 410208;湖南省中医药防治眼耳鼻咽喉疾病与视功能保护工程技术研究中心, 湖南 长沙 410208,湖南中医药大学, 湖南 长沙 410208;中医药防治眼耳鼻咽喉疾病湖南省重点实验室, 湖南 长沙 410208;湖南省中医药防治眼耳鼻咽喉疾病与视功能保护工程技术研究中心, 湖南 长沙 410208,湖南中医药大学, 湖南 长沙 410208;中医药防治眼耳鼻咽喉疾病湖南省重点实验室, 湖南 长沙 410208;湖南省中医药防治眼耳鼻咽喉疾病与视功能保护工程技术研究中心, 湖南 长沙 410208,湖南中医药大学, 湖南 长沙 410208;中医药防治眼耳鼻咽喉疾病湖南省重点实验室, 湖南 长沙 410208;湖南省中医药防治眼耳鼻咽喉疾病与视功能保护工程技术研究中心, 湖南 长沙 410208 |
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| 基金项目： | 国家自然科学基金（81573721，81874408）；湖南省自然科学基金（2016JJ6117，2019JJ40216）；2017年湖南中医药大学研究生创新项目（2017CX33）。 |
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| 摘 要： | 目的 研究益气解毒方抑制鼻咽癌CNE2细胞增殖的效应。方法 采用实时无标记细胞功能分析技术（real-time unlabeled cell function analysis technique， RTCA）动态监测不同浓度（0.125、0.250、0.500、1.000 mg/mL的益气解毒方水提物对人鼻咽癌CNE2细胞增殖的影响；采用CCK-8法检测不同浓度益气解毒方水提物对人鼻咽癌CNE2细胞增殖能力的影响；通过高通量细胞成像多功能检测系统Cytation5监测不同浓度益气解毒方水提物对鼻咽癌CNE2细胞增殖形态的影响；采用Western Blot技术检测不同浓度益气解毒方水提物对鼻咽癌CNE2细胞增殖相关蛋白Survivin、XIAP、PCNA表达的影响。结果 RTCA 和 CCK-8 法实验结果显示，与空白对照组比较，益气解毒方水提物可以明显抑制鼻咽癌CNE2细胞的增殖，而且随着益气解毒方水提物的作用时间越长，其抑制效应越显著（P<0.05）；Cytation5结果显示，与空白对照组比较，不同浓度益气解毒方水提物处理CNE2细胞后，细胞形态发生了明显变化，且浓度越高，作用时间越长，细胞膜皱缩，细胞核质浓缩、破碎更明显；Western Blot结果显示，与空白对照组比较，益气解毒方水提物能明显抑制CNE2细胞中增殖相关蛋白Survivin、XIAP、PCNA的表达（P<0.05）。结论 益气解毒方水提物能明显抑制鼻咽癌细胞增殖，该效应与其抑制增殖相关蛋白Survivin、XIAP、PCNA的表达有关。
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| 关 键 词： | 益气解毒方 鼻咽癌 细胞增殖 Survivin XIAP PCNA |
| 收稿时间： | 2019/1/31 0:00:00 |
Effects of Yiqi Jiedu Formula on Proliferation of Nasopharyngeal Carcinoma CNE2 Cells |
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| Authors: | HU Jing LIU Jie XU Bingyan FAN Jingying LUO Jingjing HU Mei and HE Yingchun |
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| Affiliation: | Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;College of Traditional Chinese Medicine, Changsha Medical University, Changsha, Hunan 410219, China,Hunan University of Chinese Medicine, Changsha, Hunan 410208, China,Hunan University of Chinese Medicine, Changsha, Hunan 410208, China,Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Traditional Chinese Medicine, Changsha, Hunan 410208, China;Hunan Provincial Ophthalmology and Otolaryngology Diseases Prevention and Treatment with Traditional Chinese Medicine and Visual Function Protection Engineering and Technological Research Center, Changsha, Hunan 410208, China,Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Traditional Chinese Medicine, Changsha, Hunan 410208, China;Hunan Provincial Ophthalmology and Otolaryngology Diseases Prevention and Treatment with Traditional Chinese Medicine and Visual Function Protection Engineering and Technological Research Center, Changsha, Hunan 410208, China,Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Traditional Chinese Medicine, Changsha, Hunan 410208, China;Hunan Provincial Ophthalmology and Otolaryngology Diseases Prevention and Treatment with Traditional Chinese Medicine and Visual Function Protection Engineering and Technological Research Center, Changsha, Hunan 410208, China and Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Traditional Chinese Medicine, Changsha, Hunan 410208, China;Hunan Provincial Ophthalmology and Otolaryngology Diseases Prevention and Treatment with Traditional Chinese Medicine and Visual Function Protection Engineering and Technological Research Center, Changsha, Hunan 410208, China |
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| Abstract: | Objective To study the inhibitory effects on the proliferation of human nasopharyngeal carcinoma CNE2 cells with Yiqi Jiedu Formula. Methods Real-time unlabeled cell function analysis technique (RTCA) was used to dynamically monitor the effects of different concentrations (0.125 mg/mL, 0.25 mg/mL, 0.5 mg/mL, 1.0 mg/mL) of Yiqi Jiedu Formula water extract on the proliferation of human nasopharyngeal carcinoma CNE2 cells; CCK-8 assay was used to detect the effects of different concentrations of water extract of Yiqi Jiedu Formula on the proliferation of human nasopharyngeal carcinoma CNE2 cells; the effects of drugs on the proliferation and morphology of nasopharyngeal carcinoma CNE2 cells were monitored by high throughput cell imaging multifunctional detection system Cytation5; Western Blot technique was used to detect the effect of drugs on the expression of proliferation-related proteins Survivin, XIAP and PCNA in nasopharyngeal carcinoma CNE2 cells. Results The results of RTCA and CCK-8 showed that the water extract of Yiqi Jiedu Formula could significantly inhibit the proliferation of nasopharyngeal carcinoma CNE2 cells compared with the blank control group, and the longer the action time of the water extract of Yiqi Jiedu Formula, the more significant the inhibitory effect was (P<0.05); Cytation5 results showed that compared with the blank control group, the morphology of CNE2 cells treated with different concentrations of Yiqi Jiedu Formula water extract changed, and the higher the concentration, the longer the action time was. The shrinkage of cell membrane, and the concentration and fragmentation of nucleoplasm were more obvious; The results of Western Blot showed that compared with the blank control group, the water extract of Yiqi Jiedu Formula could significantly inhibit the expression of proliferation-related proteins Survivin, XIAP and PCNA in CNE2 cells (P<0.05). Conclusion Yiqi Jiedu Formula can significantly inhibit the proliferation of nasopharyngeal carcinoma cells, which is related to the inhibition of the expression of proliferation-related proteins Survivin, XIAP and PCNA. |
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| Keywords: | Yiqi Jiedu Formula nasopharyngeal carcinoma cell proliferation Survivin XIAP PCNA |
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