| 旱地土壤微生物磷测定方法研究 |
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| 引用本文： | 吴金水,肖和艾,陈桂秋,黄敏.旱地土壤微生物磷测定方法研究[J].土壤学报,2003,40(1):70-78. |
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| 作者姓名： | 吴金水 肖和艾 陈桂秋 黄敏 |
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| 作者单位： | 中国科学院亚热带区域农业研究所,长沙,410125 |
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| 基金项目： | 国家杰出青年科学基金(49925102)、国家自然科学基金(49871047)和国家重点基础研究发展规划项目(G1999011802)资助 |
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| 摘 要： | 介绍了国外关于土壤微生物磷测定方法的研究进展 ,讨论了常用的几种方法所存在的问题 ,介绍了主要操作过程要求。对我国 5种主要母质类型的土壤 (pH 3 .3～ 7.4,1molL- 1KCl)的对比研究表明 ,我国土壤采用氯仿熏蒸、0 .5molL- 1NaHCO3在 1∶2 0土水比提取测定无机磷 (Pi)、并以同时测得的培养土壤微生物的磷的回收率作为计算常数得到的结果最佳。测定的大多数南方土壤的微生物磷占土壤全磷的比例小于 1 .5 % ,微生物碳磷比值大于 3 0∶1 ,反映南方土壤磷的生物活性较低 ,土壤微生物对磷的作物供应调节能力不强。
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| 关 键 词： | 土壤微生物磷 测定方法 |
| 收稿时间： | 2001/10/16 0:00:00 |
| 修稿时间： | 2001年10月16 |
MEASUREMENT OF MICROBIAL BIOMASS-P IN UPLAND SOILS IN CHINA |
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| Wu Jin-shui,Xiao He-ai,Chen Gui-qiu and Huang Min.MEASUREMENT OF MICROBIAL BIOMASS-P IN UPLAND SOILS IN CHINA[J].Acta Pedologica Sinica,2003,40(1):70-78. |
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| Authors: | Wu Jin-shui Xiao He-ai Chen Gui-qiu and Huang Min |
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| Affiliation: | The Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;The Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;The Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;The Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China |
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| Abstract: | This paper reviews previous methods for measuring the microbial biomass P in soil,with comments on their problems and limitations in the use for different soil types,and described main procedures involved in the measurement.A study was carried out to validate the methods for upland soils in China.Results show that for the 45 soils used,the flush of inorganic P(Pi)extracted in 0.5 mol L-1 NaHCO3 (pH8.5) at a soil solution ratio of 1:20 following gaseous chloroform-fumigation,as proposed by Brookes et al.4], provided a better indication of the amount of the biomass P than that extracted at a lower soil solution ratio (1:4),or in 0.03 mol L-1 NH4F-0.025 mol L-1 HCl at both the ratios(1:4 and 1:20).In most of the soils,the size of the flush of Pi was not significantly different from that of the flush of total P extracted (Pt),suggesting that the measurement of Pt was not necessary. |
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| Keywords: | Soil microbial biomass P Measuring method |
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