| 柑橘果实成熟特异基因CsPMEI/InvI的克隆与序列分析 |
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| 引用本文： | 王国立,安华明,秦巧平,李孟娇,刘真真,陈佳莹,周倩,张岚岚.柑橘果实成熟特异基因CsPMEI/InvI的克隆与序列分析[J].浙江农林大学学报,2013,30(3):336-342. |
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| 作者姓名： | 王国立 安华明 秦巧平 李孟娇 刘真真 陈佳莹 周倩 张岚岚 |
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| 作者单位： | 1.贵州省果树工程技术研究中心，贵州 贵阳 550025;2.贵州大学 农学院，贵州 贵阳 550025;3.浙江农林大学 农业与食品科学学院 浙江省农产品品质改良技术研究重点实验室，浙江 临安 311300 |
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| 摘 要： | 果实成熟特异基因对于调控果实成熟及其品质形成具有重要的作用。在前期获得柑橘Citrus果实成熟特异基因片段的基础上，以纽荷尔脐橙Citrus sinensis Newhall成熟果实为试材，应用RT-PCR和RACE技术，分离获得果实成熟特异基因的cDNA全长序列，命名为CsPMEI/InvI，GenBank登录号:KC198084；生物信息学分析表明:该基因全长945 bp，包含618 bp完整的开放阅读框，编码205个氨基酸，其编码的蛋白质分子式为C977H1568N296O282S10，相对分子量为22.29 kDa，理论等电点为9.84，属于InvI/PMEI（转化酶抑制子/果胶甲酯酶抑制子）家族成员，含有该家族严格保守的Cys残基，存在1个cAMP和cGMP-蛋白激酶磷酸化位点、3个蛋白激酶C 磷酸化位点、2个酪蛋白激酶Ⅱ磷酸化位点和3个N-酰基化位点，其二级结构主要以-螺旋为主。CsPMEI/InvI基因的分离为进一步研究柑橘果实的成熟机制提供了基础。图7表1参28
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| 关 键 词： | 园艺学 成熟特异性 果胶甲酯酶/转化酶抑制子 基因克隆 序列分析 柑橘 |
| 收稿时间： | 2012-12-26 |
Cloning and sequence analysis of fruit ripening-specific gene CsPMEI/InvI from citrus |
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| WANG Guoli,AN Huaming,QIN Qiaoping,LI Mengjiao,LIU Zhenzhen,CHEN Jiaying,ZHOU Qian,ZHANG Lanlan.Cloning and sequence analysis of fruit ripening-specific gene CsPMEI/InvI from citrus[J].Journal of Zhejiang A&F University,2013,30(3):336-342. |
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| Authors: | WANG Guoli AN Huaming QIN Qiaoping LI Mengjiao LIU Zhenzhen CHEN Jiaying ZHOU Qian ZHANG Lanlan |
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| Affiliation: | 1.Guizhou Fruits Engineering Technology Research Centre，Guiyang 550025，Guizhou，China;2.School of Agriculture，Guizhou University，Guiyang 550025，Guizhou，China;3.The Key Laboratory for Quality Improvement of Agricultural Products of Zhlejiang Province， School of Agriculture and Food Science，Zhejiang A & F University，Lin’an 311300，Zhejiang，China |
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| Abstract: | Fruit-ripening-specific expression genes play important roles on the regulation of fruit ripening and quality formation. A full-length cDNA of fruit ripening-specific gene，designated as CsPEMI/InvI （GenBank accession No. KC198084），was isolated from Newhall navel orange （Citrus sinensis Newhall） by RT-PCR and RACE. The full-length of CsPMEI/InvI was 945 bp，containing an open reading frame of 618 bp and encoding 205 amino acids. The molecular formula of encoded protein was C977H1568N296O282S10 with a calculated molecular of 22.29 kDa and an isoelectric point of 9.84. The encoded protein belonged to InvI/PMEI family including highly-conserved Cys residue in the protein sequence，one cAMP and cGMP-dependent protein kinase phosphorylation site，three protein kinase C phosphorylation sites，two casein kinase Ⅱ phosphorylation sites and three N-myristoylation sites. In its secondary structure，-helix was the main structural conformation. ［Ch，7 fig. 1 tab. 28 ref.］ |
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