| Na+ 、K+ 、pH及3种单糖对湖拟鲤精子活力的综合调控 |
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| 引用本文： | 张齐,李胜忠,邱弈树,刘长龙.Na+ 、K+ 、pH及3种单糖对湖拟鲤精子活力的综合调控[J].南方农业学报,2022,53(6):1768-1775. |
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| 作者姓名： | 张齐 李胜忠 邱弈树 刘长龙 |
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| 作者单位： | 1. 新疆农业大学生命科学学院, 新疆乌鲁木齐 830052;2. 福海县海富特种鱼养殖有限责任公司, 新疆阿勒泰 836400 |
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| 基金项目： | 新疆区域协同创新专项(科技援疆计划)(2020E0233) |
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| 摘 要： | 【目的】明确Na+、K+、pH及3种单糖对湖拟鲤精子活力的单独调控和综合调控，筛选出湖拟鲤精子的适宜激活配方，为其人工繁殖及种质资源保护提供技术参考。【方法】以快速运动时间（FT）和寿命时间（LT）为指标，采用单因素试验和正交试验研究不同渗透压条件下Na+、K+、pH及3种单糖（葡萄糖、果糖和半乳糖）对湖拟鲤精子活力的...
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| 关 键 词： | 湖拟鲤 精子活力 渗透压 正交试验 方差分析 |
| 收稿时间： | 2021-11-01 |
Comprehensive regulation of sperm motility of Rutilus rutilus lacustris by Na+,K+,pH,and 3 monosaccharides |
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| ZHANG Qi,LI Sheng-zhong,QIU Yi-shu,LIU Chang-long.Comprehensive regulation of sperm motility of Rutilus rutilus lacustris by Na+,K+,pH,and 3 monosaccharides[J].Journal of Southern Agriculture,2022,53(6):1768-1775. |
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| Authors: | ZHANG Qi LI Sheng-zhong QIU Yi-shu LIU Chang-long |
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| Affiliation: | 1. College of Life Science, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China;2. Fuhai County Haifu Special Fish Breeding Co., Ltd., Altay, Xinjiang 836400, China |
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| Abstract: | 【Objective】 To clarify the individual and comprehensive regulation of sperm motility of Rutilus rutilus lacustris by Na+，K+，pH and 3 monosaccharides，to screen the suitable formula of the sperm activating liquid of R.rutilus lacustris，so as to provide technical reference for artificial reproduction and genetic resource conservation of the R.rutilus lacustris.【Method】 Fast movement time（FT）and life time（LT）were taken as indicators，and single factor test and orthogonal test were used to study individual and comprehensive regulation effects of Na+，K+，pH and 3 monosaccharides（glucose，fructose and galactose）on sperm motility of the R.rutilus lacustris under different osmotic pressure conditions to form a suitable formula of the sperm activating liquid of R.rutilus lacustris.【Result】 When Na+ solution osmotic pressure was 282.36 kPa，the FT and LT of R.rutilus lacustris sperm reached the maximum（40.00 and 285.33 s）.When K+ solution osmotic pressure was 211.77 kPa，the FT and LT of R.rutilus lacustris sperm reached the maximum（36.00 and 130.33 s）.When glucose solution osmotic pressure was 352.95 kPa，the FT and LT of R.rutilus lacustris sperm reached the maximum（43.67 and 355.33 s）.When fructose solution osmotic pressure was 352.95 kPa，the FT and LT of R.rutilus lacustris sperm reached the maximum（45.00 and 372.67 s）.When galactose solution osmotic pressure was 235.30 kPa，the FT and LT of R.rutilus lacustris sperm reached the maximum（40.00 and 357.00 s）.When pH was 8.0，the FT and LT of R.rutilus lacustris sperm reached the maximum（30.00 and 100.33 s）.Orthogonal test results showed that the optimal formula for comprehensive regulation of sperm motility of the R.rutilus lacustris was: Fructose solution osmotic pressure of 117.65 kPa，pH of 8.0，Na+ solution osmotic pressure of 188.24 kPa，FT of 58.33 s and LT of 370.00 s.The regulation intensity was fructose>Na+>pH.The results showed that fructose and Na+had significant effects on FT and LT regulation of R.rutilus lacustris sperm（P<0.05），but the effect of pH was not significant（P>0.05）.【Conclusion】 Appropriate concentrations of Na+，K+，glucose，fructose and galactose promotes sperm motility of the R.rutilus lacustris mainly through changing osmotic pressure.The suitable pH is 7.5-8.5.Through orthogonal test，the optimal activating formula of the R.rutilus lacustris sperm is: Fructose of 117.65 kPa，pH of 8.0，and Na+ of 188.24 kPa，and regulation effect of this formula is better than that of the single-factor optimal osmotic pressure.The regulation intensity is: fructose>Na+>pH. |
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