| 黔北麻羊毛色差异皮肤组织的蛋白组学研究 |
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| 引用本文： | 张颜,孙宝盛,肖贵榜,杨玉能,杨泓涛,李世春,邓位喜.黔北麻羊毛色差异皮肤组织的蛋白组学研究[J].南方农业学报,2022,53(7):1999-2006. |
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| 作者姓名： | 张颜 孙宝盛 肖贵榜 杨玉能 杨泓涛 李世春 邓位喜 |
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| 作者单位： | 1 遵义职业技术学院, 贵州遵义 563006;2 习水县富兴牧业有限公司, 贵州遵义 564600 |
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| 基金项目： | 科技部“科技助力经济2020”重点专项（国科发资〔2020〕81号） |
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| 摘 要： | 【目的】开展黔北麻羊黑白毛色差异的蛋白组学分析，明确影响其毛色差异的特异性蛋白及其通路，为揭示黔北麻羊特征毛色的形成机制提供参考依据。【方法】分别采集3只黔北麻羊公羊背部黑色羊毛皮肤组织块和腹部白色羊毛皮肤组织块，通过SDT裂解法提取黔北麻羊皮肤组织蛋白并进行蛋白定量分析，然后对筛选出的显著差异表达蛋白分别进行GO功能注释分析、KEGG通路富集分析及亚细胞定位分析。【结果】通过组间比较共筛选出420个显著差异表达蛋白，与白色羊毛对照组（White）相比，黑色羊毛试验组（Black）有247个显著差异表达蛋白呈上调表达、173个显著差异表达蛋白呈下调表达，其中与形成黑色羊毛的黑色素相关蛋白共有15个，包括表皮视黄醇脱氢酶2（SDR16C5）、视黄醇脱氢酶16（RDH16）、视黄醇饱和酶（RETSAT）和角蛋白79（KRT79）等9个上调蛋白，以及蛋白激酶B （AKT）、β抑制蛋白1 （ARRB1）和分泌型卷曲相关蛋白1 （SFRP1）等6个下调蛋白。GO功能注释分析结果表明，显著差异表达蛋白注释到生物学过程、分子功能和细胞组分三大功能的51条GO功能条目上；亚细胞定位分析显示有159个蛋白定位于细胞质、128个蛋白定位于细胞核及88个蛋白定位于线粒体； KEGG通路富集分析结果表明，这些显著差异表达蛋白富集在209条KEGG通路上，其中5条通路与黑色素生成相关，分别是视黄醇代谢（Retinol metabolism）、黑色素生成（Melanogenesis）、丝裂原活化蛋白激酶（MAPK）、磷脂酰肌醇3激酶—蛋白激酶B（PI3K-Akt）和Wnt/β-连环蛋白（Wnt/β-catenin）通路。【结论】导致黔北麻羊存在黑白毛色差异的原因:一方面是参与黑色素生成通路的KRT79及参与视黄醇通路的SDR16C5、RDH16和RETSAT等蛋白表达上调，促进黑色素细胞中黑色素的生成和黑色羊毛的形成；另一方面是参与PI3K-AKT通路的AKT表达下调，以及SFRP1表达下调而降低对Wnt/β-catenin信号通路的抑制，均有利于黑色素生成。
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| 关 键 词： | 黔北麻羊 毛色 黑色素 皮肤组织 蛋白组学 |
| 收稿时间： | 2022-03-11 |
Proteomic analysis of skin tissue under different colors of wool of Capra hircus |
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| ZHANG Yan,SUN Bao-sheng,XIAO Gui-bang,YANG Yu-neng,YANG Hong-tao,LI Shi-chun,DENG Wei-xi.Proteomic analysis of skin tissue under different colors of wool of Capra hircus[J].Journal of Southern Agriculture,2022,53(7):1999-2006. |
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| Authors: | ZHANG Yan SUN Bao-sheng XIAO Gui-bang YANG Yu-neng YANG Hong-tao LI Shi-chun DENG Wei-xi |
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| Affiliation: | 1 Zunyi Vocational and Technical College, Zunyi, Guizhou 563006, China;2 Xishui Fuxing Animal Husbandry Co., Ltd., Zunyi, Guizhou 564600, China |
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| Abstract: | 【Objective】To carry out proteomic analysis of black and white wool color difference in Capra hircus, and to identify the specific proteins and their pathways that influenced the wool difference, so as to provide reference for revealing the mechanism of characteristic wool color of C. hircus.【Method】The back skin tissue under black wool and the abdomen skin tissue under white wool of 3 male C. hircus were detected and extracted using SDT cleavage method and analyzed quantitatively, and then significantly differential proteins were analyzed by GO function annotation, KEGG pathway enrichment and subcellular localization, respectively.【Result】By comparison, 420 differentially expressed proteins which mainly involved in biological processes, molecular functions and cell components were screened in this study. Compared with the white wool control group, 247 of the 420 significantly differential proteins in the black wool test group were up-regulated while 173 were down-regulated. Fifteen differentially expressed proteins related to melanogenesis in black wool were screened, including 9 up-regulated proteins such as epidermal retinol dehydrogenase 2(SDR16C5), retinol dehydrogenase 16(RDH16), keratin 79(KRT79), etc. and 6 down-regulated proteins such as protein kinase B (AKT), secreted frizzled related protein 1(SFRP1), etc. The results of GO function annotation analysis showed that these significantly differential proteins were annotated on 51 GO functional items of biological process, molecular function and cell component. Subcellular localization analysis showed that 159 proteins were located in cytoplasm, 128 in nucleus and 88 in mitochondria, respectively. The enrichment analysis of KEGG pathway showed that these significantly differential proteins were enriched in 209 KEGG pathways, of which 5 signal pathways were related to retinol metabolism, melanogenesis, MAPK, PI3K-Akt and Wnt/β-Catenin.【Conclusion】Reasons leading to the difference of black and white coat color in C. hircus are:For one, both the expression of KRT79 involved in the melanogenesis pathway and the expression of SDR16C5, RDH16 and RETSAT involve in the retinol pathway were up-regulated, which promotes the formation of melanin in melanocytes and the formation of black wool;for another, it is attributed to the down-regulation of AKT in PI3K-AKT pathway and the down-regulation of SFRP1 which would reduce the inhibition for Wnt/β-Catenin signaling pathway, and it is beneficial to melanin synthesis. |
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