| 福建省政和县烟草细菌性叶斑病的症状及病原菌鉴定 |
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| 引用本文： | 林可竟,周挺,詹梦琳,徐进,郑钰婷,林勇,林伟,顾钢,蔡学清.福建省政和县烟草细菌性叶斑病的症状及病原菌鉴定[J].南方农业学报,2021,52(11):3034-3040. |
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| 作者姓名： | 林可竟 周挺 詹梦琳 徐进 郑钰婷 林勇 林伟 顾钢 蔡学清 |
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| 作者单位： | 福建农林大学植物保护学院,福州 350002;福建省烟草公司烟草科学研究所,福州 350003;中国农业科学院植物保护研究所,北京 100193;福建省南平市烟草公司政和分公司,福建南平 353600;福建省南平市烟草公司,福建南平 353000 |
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| 基金项目： | 国家重点研发计划项目（2017YFD0201106-02）；中国烟草总公司福建省公司科技计划项目（KH1702210） |
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| 摘 要： | 【目的】明确福建省政和县云烟87上新发生的一种细菌性病害的病原菌，为进一步探讨其发病规律并制定有效的防控措施提供理论依据。【方法】采用平板划线分离法对采自福建省政和县的罹病云烟87烟叶进行病原菌分离纯化，对分离获得的细菌菌株通过柯赫氏法则验证其致病性，并采用生物学特征、生理生化反应及特异性引物检测、16S rDNA序列分析等明确其分类地位。【结果】从病叶上分离纯化获得15株细菌，经柯赫氏法则证明15株细菌菌株均为该病害的致病菌，且致病力无明显差异。分离菌株在NA培养基上培养1~2 d后，菌落为白色隆起，边缘不规则且黏稠，菌体短杆状，极生4根鞭毛，革兰氏染色反应阴性；经8项关键性生理生化特性测定、Biolog 94种表型测试、烟草野火病菌特异性引物检测及16S rDNA基因序列分析，将15株细菌菌株鉴定为扁桃假单胞菌烟草致病变种（Pseudomonas amygdali pv.tabaci）。【结论】引发福建省政和县烤烟叶部细菌性病害的致病菌为扁桃假单胞菌烟草致病变种。
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| 关 键 词： | 烟草 细菌性叶斑病 病原菌鉴定 扁桃假单胞菌烟草致病变种 |
| 收稿时间： | 2021-06-28 |
Symptoms and pathogen identification of tobacco bacterial leaf spot disease in Zhenghe,Fujian |
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| LIN Ke-jing,ZHOU Ting,ZHAN Meng-lin,XU Jin,ZHENG Yu-ting,LIN Yong,LIN Wei,GU Gang,CAI Xue-qing.Symptoms and pathogen identification of tobacco bacterial leaf spot disease in Zhenghe,Fujian[J].Journal of Southern Agriculture,2021,52(11):3034-3040. |
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| Authors: | LIN Ke-jing ZHOU Ting ZHAN Meng-lin XU Jin ZHENG Yu-ting LIN Yong LIN Wei GU Gang CAI Xue-qing |
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| Affiliation: | 1 College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China;2 Institute of Tobacco Science, Fujian Provincial Tobacco Company, Fuzhou 350003, China;3 Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;4 Zhenghe Branch of Nanping Tobacco Corporation, Fujian Provincial Tobacco Corporation, Nanping, Fujian 353600, China;5 Nanping Branch of Fujian Provincial Tobacco Corporation, Nanping, Fujian 353000, China |
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| Abstract: | 【Objective】 To identify the pathogenic agent of tobacco(Yunyan 87) bacterial leaf spot disease newly found in Zhenghe County of Fujian in 2020, so as to provide a theoretical basis for further exploring the disease regularity and the precise control measures.【Method】 The bacterial strains were isolated and purified from disease leaves of tobacco Yunyan 87 in Zhenghe County of Fujianby the plate-marking method, the pathogenicity of the isolated strains were tested by Koch's Postulation, and its taxonomic status were confirmed by bacteria biological characteristics, physiological and biochemical characteristics, and specific primer detection and 16S rDNA gene sequence analysis.【Result】 The results showed that fifteen strains were isolated from diseased leaves of tobacco. According to Koch's Postulation, all the fifteen strains were the pathogens of tobacco bacterial leaf spot disease and their pathogenicity was no obvious difference. The colonies of the pathogen were bulge and white on NA plate in 1-2 d cultivation, the edges were irregular and thick.The bacterial cellswere rod-shaped and had 4 flagella on polar. Gram stain was negative. Based on 8 key physiological and biochemical characteristics, 94 phenotypes in Biolog instrument, specific primer detection and 16S rDNA sequence analy-sis of the pathogens, all 15 tested isolates were identified as Pseudomonas amygdali pv. tabaci.【Conclusion】 The tobacco bacterial leaf diseases in Zhenghe County of Fujian is caused by P. amygdali pv. tabaci. |
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