Abstract: | Fifty Holstein dairy cows with palpable corpora lutea were divided into two groups. Twenty-five cows were given 500 micrograms of cloprostenol followed by 8 micrograms (2 mL) of buserelin, an analogue of gonadotropin-releasing hormone, and 25 were given cloprostenol followed by saline. Milk was collected for progesterone assay at the time of treatment and two days later. Differences in median progesterone concentrations before and following treatment were not significantly different between the saline and buserelin treated cows (p greater than 0.23). |