| 烟草尖孢镰刀菌拮抗真菌的筛选鉴定及促生作用研究 |
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| 引用本文： | 姚晨虓,李小杰,李琦,邱睿,白静科,刘畅,陈玉国,康业斌,李淑君.烟草尖孢镰刀菌拮抗真菌的筛选鉴定及促生作用研究[J].中国生物防治学报,2021,37(5):1066-1072. |
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| 作者姓名： | 姚晨虓 李小杰 李琦 邱睿 白静科 刘畅 陈玉国 康业斌 李淑君 |
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| 作者单位： | 1. 河南科技大学园艺与植物保护学院, 洛阳 471023;2. 烟草行业黄淮烟区烟草病虫害绿色防控重点实验室/河南省农业科学院烟草研究所, 许昌 461000;3. 中国烟草总公司河南省公司, 郑州 450018 |
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| 基金项目： | 河南省烟草公司科技项目（201741000027063，2020410000270012） |
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| 摘 要： | 为获得烟草镰刀菌根腐病拮抗真菌菌株，试验以河南省豫中烟区烟草根际土壤为试材，以主要致病菌尖孢镰刀菌Fusarium oxysporum为主要靶标，采用稀释涂布平板法分离获得土壤真菌371株，平板对峙培养法测定获得1株具有明显抑制效果的拮抗菌株，利用形态学和分子生物学的方法将其鉴定为棘孢木霉Trichoderma asperellum Tr-0111。抑制效果测定表明，该菌株对尖孢镰刀菌的抑制率最高可达93.13%，对根串珠霉菌、拟茎点霉、链格孢菌等其他8种烟草病原菌均具有较强的抑制效果。促生作用测定结果表明，菌株发酵液使烟草种子萌发率提高5.34%，烟苗根长增长率为62.39%，对盆栽烟草的最大叶面积和地上部鲜重也具有显著的促生效果。为进一步相关机理的研究和生防菌剂的研制奠定了基础。
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| 关 键 词： | 烟草镰刀菌根腐病 尖孢镰刀菌 棘孢木霉 抑制效果 促生作用 |
| 收稿时间： | 2021-01-06 |
Screening and Identification of Antagonistic Fungal against Tobacco Fusarium oxysporum and Its Growth Promotion Effect |
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| YAO Chenxiao,LI Xiaojie,LI Qi,QIU Rui,BAI Jingke,LIU Chang,CHEN Yuguo,KANG Yebin,LI Shujun.Screening and Identification of Antagonistic Fungal against Tobacco Fusarium oxysporum and Its Growth Promotion Effect[J].Chinese Journal of Biological Control,2021,37(5):1066-1072. |
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| Authors: | YAO Chenxiao LI Xiaojie LI Qi QIU Rui BAI Jingke LIU Chang CHEN Yuguo KANG Yebin LI Shujun |
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| Affiliation: | 1. Horticulture and Plant Protection College, Henan University of Science and Technology, Luoyang 471023, China;2. Key Laboratory for Green Preservation & Control of Tobacco Diseases and Pests in Huanghuai Growing Area/Tobacco Research Institute of Henan Academy of Agricultural Sciences, Xuchang 461000, China;3. Henan Provincial Tobacco Company, Zhengzhou 450018, China |
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| Abstract: | In order to obtain the antagonistic fungal strains against tobacco Fusarium root rot, 371 fungal strains were isolated from the tobacco rhizosphere soil in the tobacco area of Henan Province by dilution coating plate method. One Trichoderma strain with obvious inhibitory effect against F. oxysporum was obtained by the plate confrontation culture method, and was identified as T. asperellum Tr-0111 by morphological and molecular biology methods. Tr-0111 strain had a maximum inhibitory rate of 93.13% against F. oxysporum, and also had a strong inhibitory effect on 8 other tobacco pathogens, such as Thielaviopsis basicola, Phomopsis sp., Alternaria alternata, etc. The growth-promoting effect test showed that the fermentation broth of the strain increased the germination rate of tobacco seeds by 5.34%, and the increase rate of root length of tobacco seedlings was 62.39%. It also had a significant growth-promoting effect on the maximum leaf area and fresh weight of the above-ground part of potted tobacco. The results laid the foundation for further research on the related mechanisms and development of biocontrol agents. |
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| Keywords: | Tobacco Fusarium root rot Fusarium oxysporum Trichoderma asperellum inhibitory effect growth promotion |
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