| 接种摩西柄囊霉对连作大豆异黄酮及脂肪酸含量的影响 |
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| 引用本文： | 崔佳琦,魏丽娜,蔡柏岩.接种摩西柄囊霉对连作大豆异黄酮及脂肪酸含量的影响[J].农学学报,2015,5(4):31-35. |
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| 作者姓名： | 崔佳琦 魏丽娜 蔡柏岩 |
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| 作者单位： | 黑龙江大学,黑龙江大学生命科学学院,黑龙江大学生命科学学院 |
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| 基金项目： | 黑龙江省教育厅面上资助项目“连作大豆土壤AM真菌菌群结构分析及其与根腐病病原菌相互关系研究”(12521394) |
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| 摘 要： | 为探索接种摩西柄嚢霉(F. mosseae)对连作大豆籽粒异黄酮及脂肪酸含量的影响，为把F. mosseae应用于连作大豆的生产提供理论依据。试验选用‘垦丰16’（中间型品种，KF16）、‘黑农44’（高脂肪型品种，HN44）和‘黑农48’（高蛋白型品种，HN48）作为试验材料。试验采用盆栽，每盆装风干土12.5 kg，土壤为连作0年(CK)、1年(L1)、2年(L2)的大豆大田土壤。每盆接种F. mosseae菌剂200 g。采用紫外分光光度计法和气相色谱法，对成熟大豆籽粒中异黄酮以及脂肪酸含量进行测定。结果表明：KF16、HN44和HN48分别在L1，L2，L2时，异黄酮含量最高。KF16、HN44和HN48脂肪酸含量随连作年限增加而降低。接种F. mosseae可以提高大豆异黄酮含量，降低脂肪酸含量。
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| 关 键 词： | 酵母菌 酵母菌 谷胱甘肽 筛选 提取 正交试验 |
| 收稿时间： | 2014/9/22 0:00:00 |
| 修稿时间： | 2014/10/27 0:00:00 |
Effect of Inoculated Funneliformis mosseae on the Content of Isoflavone and Fatty Acid in the Continuous Cropping of Soybean |
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| Abstract: | The study aim to explore the effect of inoculated F. mosseae on the content of isoflaone and fatty acid. in order to provide the theoretical evidence using F. mosseae to the continuous cropping. Three soybean cultivars KF16 (middle cultivar), HN44 (high-oil cultivar), HN48 (high-protein cultivar) were investigated. The soybean seeds use 0-year soil samples (CK), 1-year soil samples (L1) and 2-year soil samples (L2) planted in the pot with 12.5 kg dry soil sample and inoculated 200g F. mosseae into each pot. The accumulation of the mature soybean isoflavone and fatty acid was determined by ultraviolet spectrophotometry and gas chromatography. It showed that: the highest isoflavone content of KF16, HN44 and HN48 was L1, L2 and L2, respectively. And with the increase of continuous cropping, the fatty acid content of KF16, HN44 and HN48 is decreasing. Inoculated F. mosseae could increase the content of isoflavone and decrease the content of fatty acid. |
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| Keywords: | soybean F mosseae continuous cropping isoflavone fatty acid content |
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