| 基于代谢组解析大黄鱼对低温和饥饿胁迫的适应机制 |
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| 引用本文： | 曾霖,宋炜,谢正丽,王永红,熊逸飞,张惠.基于代谢组解析大黄鱼对低温和饥饿胁迫的适应机制[J].水产学报,2023,47(7):079608-1-079608-12. |
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| 作者姓名： | 曾霖 宋炜 谢正丽 王永红 熊逸飞 张惠 |
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| 作者单位： | 蚌埠学院食品与生物工程学院;浙江海洋大学国家海洋设施养殖工程技术研究中心,中国水产科学研究院东海水产研究所,中国水产科学研究院渔业机械仪器研究所,蚌埠学院食品与生物工程学院,中国水产科学研究院东海水产研究所,浙江海洋大学国家海洋设施养殖工程技术研究中心;浙江海洋大学国家海洋设施养殖工程技术研究中心 |
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| 基金项目： | 科技部国家重点研发计划——蓝色粮仓科技创新专项(2019YFD0900904)；青岛海洋科学与技术试点国家实验室山东省专项经费(2022QNLM30001)；财政部和农业农村部国家现代农业产业技术体系资助(CARS-47)；中国水产科学研究院基本科研业务费(2020TD76) |
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| 摘 要： | 为探讨大黄鱼Larimichthys crocea对低温和饥饿氧化损伤的响应机制，本实验将体质量为（21.38±2.46）g的大黄鱼在低温（8°C）或/和禁食条件下饲养。实验组可分为4个处理组：对照组（C组）、低温组（CC组）、饥饿组（F组）和饥饿+低温组（CF组），每组3个平行。低温和饥饿胁迫30 d后，计算成活率；采取肝脏样本，进行组织学观察，并利用化学荧光法和LC-MS非靶向代谢组学技术分析处理组间活性氧（ROS）和代谢产物的差异。结果表明，与C组相比，CC组、F组和CF组的成活率显著降低，而ROS含量显著升高（P < 0.05），且肝细胞均出现不同程度的空泡和核萎缩现象，表明低温和饥饿胁迫对大黄鱼产生了氧化损伤。大黄鱼低温应激后，从CC vs. C和CF vs. F中分别筛选出84种和154种差异代谢物，有5种重要的重叠代谢途径：甘油磷脂代谢、糖基磷脂酰肌醇（GPI）-锚生物合成和自噬等，表明细胞膜流动性和自噬在大黄鱼低温适应过程中发挥重要作用。大黄鱼饥饿应激后，从F vs. C和CF vs. CC中分别筛选出184种和50种差异代谢物，有4种重要的重叠代谢途径：甘油磷脂代谢、糖基磷脂酰肌醇（GPI）-锚生物合成、ABC运输体和自噬等，表明能量代谢和自噬在大黄鱼饥饿过程中发挥重要作用。从CF vs. C中筛选出差异代谢物126种，主要富集在糖基磷脂酰肌醇（GPI）-锚生物合成、甘油磷脂代谢、氧化磷酸化、淀粉和蔗糖代谢、FoxO信号通路、自噬和谷胱甘肽代谢等，表明细胞膜流动性、能量代谢、自噬和抗氧化系统在大黄鱼适应低温和饥饿联合胁迫过程中发挥重要作用。本文结果为深入研究低温及其诱导的饥饿对大黄鱼生理功能的影响提供科学依据。
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| 关 键 词： | 大黄鱼、低温、饥饿、代谢组学 |
| 收稿时间： | 2022/10/28 0:00:00 |
| 修稿时间： | 2023/2/28 0:00:00 |
Metabolomics-based analysis of adaptive mechanism of Larimichthys crocea to low temperature and starvation stresses |
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| Lin ZENG,Wei SONG,Zhengli XIE,Yonghong WANG,Yifei XIONG,Hui ZHANG.Metabolomics-based analysis of adaptive mechanism of Larimichthys crocea to low temperature and starvation stresses[J].Journal of Fisheries of China,2023,47(7):079608-1-079608-12. |
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| Authors: | Lin ZENG Wei SONG Zhengli XIE Yonghong WANG Yifei XIONG Hui ZHANG |
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| Affiliation: | School of Food and Biological Engineering,Bengbu University;China;;East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences;China;;National Engineering Research Center of Marine Facilities Aquaculture,Zhejiang Ocean University;China;,East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,songw@ecsf.ac.cn,School of Food and Biological Engineering,Bengbu University,East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,National Engineering Research Center of Marine Facilities Aquaculture,Zhejiang Ocean University |
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| Abstract: | Large yellow croaker Pseudosciaena crocea is the most important warm-water marine commercial fish, which is widely distributed in the southeast coastal regions in China, such as Zhejiang, Fujian and Guangdong provinces. It is highly sensitive to cold and starvation, and the mass mortality of this species was observed during the over-winter period, leading to significant economic losses for farmers. This study was to explore the response mechanism of large yellow croaker to oxidative damage caused by low temperature and starvation. Fish with an average weight of (21.38±2.46) g was exposed to low temperature (8°C) or/and under starvation stress. Fish was divided into four groups: control group (C group), cold group (CC group), fasting group (F group) and cold+fasting group (CF group), each group with three replicates. The experiment lasted30 d, survival rate was calculated, liver samples were obtained for histological observation, chemical fluorescence and LC-MS technology were used to analyze the difference of reactive oxygen species (ROS) and metabolites in different treatment groups. The results showed that compared with C group, the survival rate of CC group, F group and CF group was significantly decreased, while the ROS content was significantly increased (P<0.05), and demonstrated vacuolation and nuclear atrophy, indicating cold and starvation stresses induced oxidative damage. A total of 184 and 50 differential metabolites were obtained from CC vs. C and CF vs. F, respectively. There were five important co-metabolic pathways: glycerophospholipid metabolism, glycosylphosphatidylinositol (GPI) - anchor biosynthesis, arginine biosynthesis, sphingolipids metabolism and autophagy, indicating cell membrane fluidity and autophagy played an important role in the cold adaptation. A total of 184 and 50 different metabolites were obtained from F vs. C and CF vs. CC, respectively. There were four important overlapping metabolic pathways: glycerophospholipid metabolism, glycosylphosphatidylinositol (GPI) - anchor biosynthesis, ABC transporters and autophagy, indicating energy and material transport function, and autophagy played an important role in the starvation stress of large yellow croaker. 126 differential metabolites were obtained from CF vs. C, which were significantly enriched in glycosylphosphatidylinositol (GPI) - anchor biosynthesis, glycerophospholipid metabolism, oxidative phosphorylation, starch and sucrose metabolism, FoxO signal pathway, autophagy and glutathione metabolism, indicating cell membrane fluidity, energy metabolism, autophagy and antioxidant systems played an important role in the cold and fasting adaptation. The results provided scientific basis for further study about the effects of low temperature and fasting on the physiological functions of large yellow croaker. |
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| Keywords: | Pseudosciaena crocea low temperature starvation metabonomics |
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