| 矮牵牛的查尔酮合酶(CHS)基因的生物信息学分析 |
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| 引用本文： | 汪结明,王凤玲,李瑞雪,魏万亮.矮牵牛的查尔酮合酶(CHS)基因的生物信息学分析[J].农学学报,2011(4). |
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| 作者姓名： | 汪结明 王凤玲 李瑞雪 魏万亮 |
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| 作者单位： | 湖南科技大学生命科学学院; |
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| 基金项目： | 湖南省教育厅一般项目“湖南刚竹属竹种遗传多样性的ISSR分析的研究”(10C0718) |
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| 摘 要： | 为研究CHS(chalcone synthase)基因间的同源性及其进化关系,以矮牵牛的CHS基因为研究对象,利用生物信息学软件及网站对其进行碱基分布、氨基酸组成和亲疏水性预测,并与其他10个物种的CHS基因序列进行多重比对、进化分析。结果表明:矮牵牛的CHS基因的单链mRNA序列含有1170个碱基对,蛋白质由389个氨基酸编码,相对分子质量为42580.02,其中亮氨酸(Leu)含量最高达10.80%;疏水值最大为2.038,最小为-2.208,无明显的亲水或疏水区域;多重比对发现11个物种同源性达到了80.55%。通过以上结果得出CHS基因处于稳定状态,编码的蛋白为亲水性蛋白,进化过程中是保守的,获得的保守区段的序列信息为新基因的克隆奠定了基础。
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| 关 键 词： | 查尔酮合成酶基因 花色 生物信息学 序列分析 |
Analysis on Bioinformatics of Chalcone Synthetase Gene in Petunia hybrida Vilm. |
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| Wang Jieming,Wang Fengling,Li Ruixue,Wei Wanliang.Analysis on Bioinformatics of Chalcone Synthetase Gene in Petunia hybrida Vilm.[J].Journal of Agriculture,2011(4). |
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| Authors: | Wang Jieming Wang Fengling Li Ruixue Wei Wanliang |
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| Affiliation: | Wang Jieming,Wang Fengling,Li Ruixue,Wei Wanliang ( School of Life Sciences,Hunan University of Science and Technology,Xiangtan 411201,Hunan,China) |
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| Abstract: | To study the homology and evolutionary relationships among CHS (chalcone synthase) genes, and using the CHS gene in Petunia hybrida as studied object, its base distribution, amino acids composition and hydrophobicity or hydrophilicity were analyzed by the software and bioinformatics web site, as well as multiple comparisons and phylogenetic analysis with other 10 species. The mRNA single sequence contained 1170 bp base, the number of amino acids encoding protein was 389 and its relative molecular mass was 4... |
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| Keywords: | Chalcone Synthetase Gene Flower Colour Bioinformatics Sequence Analysis |
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