Immune synergistic oligodeoxynucleotide from Lactobacillus rhamnosus GG enhances the immune response upon co‐stimulation by bacterial and fungal cell wall components |
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| Authors: | Shireen Nigar Yoshinari Yamamoto Takuma Okajima Takashi Sato Tasuku Ogita Takeshi Shimosato |
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| Affiliation: | 1. Interdisciplinary Graduate School of Science and Technology, Shinshu University, Minamiminowa, Nagano, Japan;2. Department of Nutrition and Food Technology, Jessore University of Science and Technology, Jessore, Bangladesh;3. Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Hiroshima, Japan;4. Department of Agricultural and Life Science, Graduate School of Science and Technology, Shinshu University, Minamiminowa, Nagano, Japan;5. Department of Pulmonology, Graduate School of Medicine, Yokohama City University, Yokohama, Japan;6. Institute for Biomedical Sciences, Shinshu University, Minamiminowa, Nagano, Japan;7. Research Center for Fungal and Microbial Dynamism, Minamiminowa, Nagano, Japan |
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| Abstract: | Bacterial genomic DNA has recently been shown to elicit a highly evolved immune defense. This response can be selectively triggered for a wide range of therapeutic applications, including use as a vaccine adjuvant to immunotherapies for allergy, cancer, and infectious diseases. Previously, we identified a low‐concentration immune synergistic oligodeoxynucleotide (iSN‐ODN, named iSN34) from Lactobacillus rhamnosus GG that has immunosynergistic activity upon costimulation of target cells with ligands of Toll‐like receptor 9 (TLR9). Here, we extend that observation by demonstrating the synergistic induction (in mouse splenocytes) of IL‐6 by the combination of iSN34 with cell wall components of bacteria and fungi. We observed that splenocytes pretreated with iSN34 and then costimulated with agonists for TLR1/2 (Pam3CSK4), TLR4 (lipopolysaccharide), or TLR2/6 (Zymosan) exhibited enhanced accumulation of IL‐6. These results suggested that the combination of iSN34 with TLR1/2, TLR4, or TLR2/6 agonists may permit the induction of a potent immune response. |
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| Keywords: | IL‐6 immune‐synergistic oligodeoxynucleotide LPS Pam3CSK4 Zymosan |
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