| 大珠母贝基因转移的电击参数 |
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| 引用本文： | 喻达辉.大珠母贝基因转移的电击参数[J].水产学报,2001,25(5):408-412. |
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| 作者姓名： | 喻达辉 |
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| 作者单位： | 1. 中国水产科学研究院南海水产研究所,
2. 中国科学院水生生物研究所, |
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| 基金项目： | 广东省自然科学基金(991044)和淡水生态与生物技术国家重点实验室资助项目 |
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| 摘 要： | 将大珠母贝精子经不同脉冲循环数、脉冲数和不同脉冲幅度处理后，与卵子受精，然后观察受精和发育情况并进行统计分析，根据受精率确定最佳参数组合为：脉冲循环数6、脉冲数2^8和脉冲幅度6KV。此前用电击参数为脉冲循环数6、脉冲数2^7和脉冲幅度10KV进行了大珠母贝转基因实验，获得转基因阳性贝。
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| 关 键 词： | 大珠母贝 基因转移 电脉冲 电击参数 |
| 文章编号： | 1000-0615-(2001)05-0408-05 |
| 收稿时间： | 2014/4/14 0:00:00 |
| 修稿时间： | 2000年3月21日 |
Parameters of electroporation in transgene of Pinctada maxima |
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| YU Da-hui.Parameters of electroporation in transgene of Pinctada maxima[J].Journal of Fisheries of China,2001,25(5):408-412. |
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| Authors: | YU Da-hui |
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| Affiliation: | South China Sea Fisheries Research Institute, CAFS, Guangzhou 510300, China; Institute of Hydrobiology, CAS, Wuhan 430072, China |
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| Abstract: | The sperms of Pinctada maxima were electroporated with different parameters of electric pulses: changing cycles of pulses, numbers of pulses in one cycle, or amplitude of pulse. The treated sperms were fertilized with the eggs subsequently. The fertilization and development were observed and the ratios of fertilization were statistically counted, by which the optimal combination of these three parameters was determined. The results were as follows: cycles of pulses being 6, numbers of pulses in one cycle being 28, amplitude of pulse being 6kv. Before this experiment, the gene transfer was conducted through electroporation with the parameters of cycles of 6, numbers of pulses of 27 in one cycle and amplitude of 10kv, and positive transgenic pearl oysters were obtained. The parameters were basically indentical with those of this study. The application further testified the fitness of parameters quantified by the rates of fertilization in this study. |
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| Keywords: | Pinctada maxima gene transfer electric pulse parameter of electroporation |
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