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Growth,feed conversion and body composition of fingerling stinging catfish Heteropneustes fossilis (Bloch) fed varying levels of dietary l‐threonine
Authors:  Farhat  Mukhtar A Khan
Affiliation:Fish Nutrition Research Laboratory, Department of Zoology, Aligarh Muslim University, Aligarh, India
Abstract:An 8‐week feeding trial was conducted to assess the effects of dietary l ‐threonine on growth, protein utilization, threonine retention efficiencies, nucleic acid indices and body composition of fingerling Heteropneustes fossilis (6.6 ± 0.1 g; 10.9 ± 0.2 cm). Casein–gelatin based isonitrogenous (38% crude protein; CP) and isocaloric (15.3 kJ g?1 digestible energy; DE) amino acid test diets with six levels of dietary l ‐threonine (0.75%; 1.0%; 1.25%; 1.5%; 1.75%; 2.0% dry diet) were prepared and hand‐fed to quadruplicate groups of fingerling to apparent visual satiation twice daily. Weight gain (WG; 46.3 g fish?1), feed conversion ratio (FCR; 1.98), protein utilization efficiency (PUE; 0.25), threonine retention efficiency (TRE; 0.69), lipid productive value (LPV; 0.45), body protein (18.2%) and RNA/DNA ratio (3.6) of fish fed graded levels of dietary threonine increased significantly (P < 0.05) up to 1.49% threonine of dry diet. To generate precise information, the WG, RNA/DNA and LPV data were subjected to broken‐line and quadratic regression analyses. The two models were superimposed and requirement was determined by establishing the point, where the quadratic curve first intersected the plateau of broken‐line. Based on the above mathematical analyses, optimum dietary threonine requirement of fingerling Hfossilis was estimated to range between 1.62% and 1.69% of the diet, corresponding to 4.26–4.44% protein.
Keywords:threonine  growth  fingerling  H     fossilis
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