| 四川省部分地区肉牛源产超广谱β-内酰胺酶肺炎克雷伯菌的分离鉴定与耐药性分析 |
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| 引用本文： | 李姗,黄方园,张玉龙,才冬杰,左之才.四川省部分地区肉牛源产超广谱β-内酰胺酶肺炎克雷伯菌的分离鉴定与耐药性分析[J].浙江农业学报,2022,34(5):923. |
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| 作者姓名： | 李姗 黄方园 张玉龙 才冬杰 左之才 |
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| 作者单位： | 四川农业大学 动物医学院,四川 温江 611130 |
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| 基金项目： | 国家重点研发计划(2018YFD0501800);四川省科技计划(2018NZ0002);四川省科技计划(2019YFQ0012);国家现代农业产业技术体系四川肉牛创新团队(SCCXTD-2020-13) |
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| 摘 要： | 从四川省15个肉牛养殖场采集222份鼻腔拭子样本,采用CHROMagar ESBL显色培养基,利用16S rRNA进行细菌鉴定,并通过双纸片协同验证产超广谱β-内酰胺酶(ESBLs)肺炎克雷伯菌,用纸片扩散法(Kirby-Bauer法)进行药物敏感试验,利用PCR检测产ESBLs耐药基因、荚膜血清型和毒力基因,并进行小鼠致病性试验。结果显示,从222份样本中共检出产ESBLs肺炎克雷伯菌16株(7.21%)。16株分离株均表现出多重耐药特征,主要对苯唑西林、阿莫西林、四环素、链霉素耐药,耐药率在62.50%~93.75%,对亚胺培南敏感。16株分离株全部携带氨基糖苷类aac(3)-Ⅱ、aac(6')-Ⅰb、四环素类tetA、链霉素strA、磺胺类sul1、sul2基因,14株(87.50%)携带aph(3')-Ⅰa基因,11株(68.75%)携带strB基因,仅一株携带aac(3)-Ⅰ基因。16株分离株中,荚膜血清型检出5株(31.25%),包括3株K5、1株K1、1株K20。小鼠致病性试验结果显示,16株分离株均有较强的致病性,观察病理组织发现,肺炎克雷伯菌对小鼠肺损害严重。综上,从四川地区分离的16株产ESBLs肺炎克雷伯菌呈现多重耐药特征,强毒力菌株以K5荚膜型为主,提示临床应进一步加强耐药监测,合理使用抗菌药物,以防止多重耐药菌株和强毒力菌株的传播与流行。
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| 关 键 词： | 肉牛源 超广谱β-内酰胺酶 肺炎克雷伯菌 耐药特征 |
| 收稿时间： | 2021-05-06 |
Isolation,identification and drug resistance of extended spectrum β-lactamases (ESBLs) producing Klebsiella pneumoniae from beef cattle in Sichuan Province,China |
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| LI Shan,HUANG Fangyuan,ZHANG Yulong,CAI Dongjie,ZUO Zhicai.Isolation,identification and drug resistance of extended spectrum β-lactamases (ESBLs) producing Klebsiella pneumoniae from beef cattle in Sichuan Province,China[J].Acta Agriculturae Zhejiangensis,2022,34(5):923. |
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| Authors: | LI Shan HUANG Fangyuan ZHANG Yulong CAI Dongjie ZUO Zhicai |
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| Affiliation: | College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China |
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| Abstract: | In the present study, 222 nasal swabs were collected from 15 beef cattle farms in Sichuan Province, China. The extended spectrum β-lactamases (ESBLs)producing Klebsiella pneumoniae was identified by using CHROMagar ESBL chromogenic medium and 16S rRNA. Kirby Bauer method was used for drug sensitivity test. PCR was used to detect ESBLs resistant genes, capsular serotype and virulence genes, and pathogenicity test was carried out in mice. The results showed that, a total of 16 strains (7.21%) of ESBLs producing K. pneumoniae were detected from 222 samples. The 16 strains showed multi drug resistance, mainly to oxacillin, amoxicillin, tetracycline and streptomycin, with drug resistance rate of 62.50%-93.75%, and were sensitive to imipenem. All the 16 strains carried aminoglycoside aac(3)-Ⅱ,aac(6')-Ib,tetracycline tetA, streptomycin strA,sulfonamide sul1 and sul2 genes, 14 strains (87.50%) carried aph(3')-Ⅰa gene, 11 strains (68.75%) carried strB gene,and only one strain carried aac(3)-Ⅰ gene. Among the 16 strains, 5 (31.25%) were capsular serotypes, and the quantity for K5, K1 and K20 was 3, 1, 1, respectively. All the 16 strains had strong pathogenicity, which caused serious damage to the lung of mice. It was concluded that the isolated 16 strains of ESBLs producing K. pneumoniae in Sichuan Province showed multi drug resistance. The most virulent strains were K5 capsular type. Therefore, clinical drug resistance monitoring should be further strengthened, and antibiotics should be used reasonably to prevent the spread and prevalence of multi drug resistant strains and high virulent strains of K. pneumoniae. |
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| Keywords: | beef cattle extended spectrum β-lactamase Klebsiella pneumoniae drug resistance characteristics |
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