| 人参黑斑病生防菌的分离鉴定及防效 |
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| 引用本文： | 张宁,张晶晶,李雅淑,李冉琪,侯微,曲正义,李亚丽,郑培和.人参黑斑病生防菌的分离鉴定及防效[J].中国生物防治学报,2022,38(5):1308-1315. |
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| 作者姓名： | 张宁 张晶晶 李雅淑 李冉琪 侯微 曲正义 李亚丽 郑培和 |
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| 作者单位： | 1. 中国农业科学院特产研究所, 长春 130112;2. 吉林农业科技学院, 吉林 132109 |
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| 基金项目： | 国家重点研发计划(2021YFD1600900、2017YFC1702102);中国农业科学院科技创新工程(CAAS-ASTIP-2016-ISAPS) |
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| 摘 要： | 本研究以人参链格孢为靶标菌，采用平板对峙法从人参根际土壤筛选出一株拮抗细菌YJ 3-7，结合细胞形态判断、16S rDNA和gyrA基因序列比对分析，鉴定菌株YJ 3-7为贝莱斯芽胞杆菌Bacillus velezensis。该菌株的发酵液、菌悬液和无菌发酵液对人参链格孢的生长均具有抑制作用，抑制率分别为98.76%、98.27%和70.62%。离体叶片和盆栽试验表明YJ 3-7发酵液可以较好地防治人参黑斑病，防效分别为63.24%和67.92%，盆栽防效略低于多菌灵(72.81%)。除此之外，菌株YJ 3-7还能有效抑制其他8种病原真菌的生长。研究表明贝莱斯芽胞杆菌YJ 3-7抑菌谱广，对人参黑斑病具有显著的防治效果，在植物病害防治中具有一定的应用潜力，可为人参黑斑病生物防治药剂的开发奠定基础。
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| 关 键 词： | 人参黑斑病 贝莱斯芽胞杆菌 生物防治 |
| 收稿时间： | 2022-05-04 |
Isolation,Identification and Control Effect of Biocontrol Bacteria against Ginseng Black Spot Disease |
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| ZHANG Ning,ZHANG Jingjing,LI Yashu,LI Ranqi,HOU Wei,QU Zhengyi,LI Yali,ZHENG Peihe.Isolation,Identification and Control Effect of Biocontrol Bacteria against Ginseng Black Spot Disease[J].Chinese Journal of Biological Control,2022,38(5):1308-1315. |
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| Authors: | ZHANG Ning ZHANG Jingjing LI Yashu LI Ranqi HOU Wei QU Zhengyi LI Yali ZHENG Peihe |
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| Affiliation: | 1. Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China;2. Jilin Agricultural Science and Technology University, Jilin 132109, China |
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| Abstract: | An antagonistic bacterial strain YJ 3-7 was screened from the rhizosphere soil of ginseng by plate confrontation method using Alternaria panax as indicators.Combined with cell morphology,16S rDNA and gyrA gene sequence alignment analysis,the strain YJ 3-7 was identified as Bacillus velezensis.Fermentation broth,bacterial suspension and fermentation liquid without bacteria of strain YJ 3-7 all had inhibitory effects on the growth of A.panax,and the inhibition rates were 98.76%,98.27% and 70.62%,respectively.In vitro leaf experiment and pot experiment showed that YJ 3-7 fermentation broth could control ginseng black spot disease,with the control efficacies of 63.24% and 67.92%,respectively.The pot control effect of YJ 3-7 fermentation broth was slightly lower than that of carbendazim (72.81%).In addition,strain YJ 3-7 could effectively inhibit the growth of other 8 plant pathogens.The above results show that B.velezensis YJ 3-7 has broad-spectrum antagonistic effect,and obvious control effect on ginseng black spot disease,which laid a foundation for the development of bio-control agents against ginseng black spot disease. |
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| Keywords: | ginseng black spot Bacillus velezensis biological control |
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