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Methane production from thermophilic co‐digestion of dairy manure and waste milk obtained from therapeutically treated cows
Authors:Nilmini Beneragama  Masahiro Iwasaki  Kazutaka Umetsu
Affiliation:1. Department of Environmental Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan;2. Agricultural Biotechnology Center, Faculty of Agriculture, University of Peradeniya, Peradeniya, Sri Lanka
Abstract:Methane production from co‐digestion of dairy manure and waste milk, milk from cows treated with antibiotics for mastitis, was tested in a 2 × 4 factorial design. Four different waste milk percentages (w/w): 0% (SM), 10% (SMWM10), 20% (SMWM20) and 30% (SMWM30), were tested with two slurry percentages (w/w): 50% (A) and 25% (B) and the rest being manure at 55°C for 12 days in batch digesters. The results analyzed using a Gompertz model showed SMWM10 produced the highest methane production potential (Pm)/g volatile solids added followed by SM in both A and B. This Pm of SMWM10 in A and B was statistically non‐significant (P > 0.05). More than 96% of cefazolin‐resistant bacteria and 100% of multi‐drug‐resistant bacteria reductions were observed in all the treatments. Inclusion of waste milk at 10% in single stage digester enhances the methane production from dairy manure and could offer added benefit of waste milk treatment and disposal.
Keywords:cefazolin‐resistant bacteria  co‐digestion  dairy manure  methane  waste milk
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