| 虫生广布拟盘多毛孢侵染对松突圆蚧海藻糖酶 活性、海藻糖、葡萄糖和蛋白质含量的影响 |
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| 引用本文： | 王莎莎,何锦华,罗世念,彭锋,罗林,黄宝灵,吕成群.虫生广布拟盘多毛孢侵染对松突圆蚧海藻糖酶 活性、海藻糖、葡萄糖和蛋白质含量的影响[J].南方农业学报,2017,48(5):820-824. |
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| 作者姓名： | 王莎莎 何锦华 罗世念 彭锋 罗林 黄宝灵 吕成群 |
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| 作者单位： | 广西大学 林学院,南宁,530004玉林市森林病虫防治检疫站,广西 玉林,537000玉林市玉州区森林病虫防治检疫站,广西 玉林,537000 |
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| 基金项目： | 国家自然科学基金项目，广西林业科技项目 |
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| 摘 要： | 目的]探究虫生广布拟盘多毛孢对松突圆蚧营养物质代谢的影响,为深入研究广布拟盘多毛孢致死松突圆蚧机理及开发利用广布拟盘多毛孢防治松突圆蚧提供科学依据.方法]以虫生广布拟盘多毛孢GXSTYJ03菌株制得浓度为1×107个孢子/mL的孢子悬浮液感染松突圆蚧,以喷洒无菌吐温-80为对照,逐日测定松突圆蚧体内海藻糖、葡萄糖、蛋白质含量及海藻糖酶活性,连续测定8 d.结果]处理组松突圆蚧海藻糖含量在各时间点均低于对照组,且随处理时间延长呈明显的下降趋势,在染菌3 d后显著低于对照组(P<0.05,下同);海藻糖酶活性在各时间点均高于对照组,在染菌后第3 d显著高于对照组,5 d后也一直显著高于对照组;葡萄糖含量在染菌后1~3 d迅速升高,第3 d时升至最大值(3.1479 mmol/L),随后逐渐降低,在第4~6 d时与对照组无显著差异(P>0.05),6 d后显著低于对照组;蛋白质含量在各时间点均低于对照组,且在染菌2 d后一直显著或极显著(P<0.01)低于对照组.结论]虫生广布拟盘多毛孢GXSTYJ03菌株侵染松突圆蚧过程中引起松突圆蚧体内海藻糖、葡萄糖和蛋白质含量一定程度降低,是致死松突圆蚧的原因之一.
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| 关 键 词： | 虫生广布拟盘多毛孢 松突圆蚧 海藻糖 葡萄糖 海藻糖酶 蛋白质 |
Influence of Entomogenous Pestalotiopsis disseminata on trehalase activity,trehalose content,glucose content and protein content in Hemiberlesia pitysophila Takagi |
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| WANG Sha-sha,HE Jin-hua,LUO Shi-nian,PENG Feng,LUO Lin,HUANG Bao-ling,LYU Cheng-qun.Influence of Entomogenous Pestalotiopsis disseminata on trehalase activity,trehalose content,glucose content and protein content in Hemiberlesia pitysophila Takagi[J].Journal of Southern Agriculture,2017,48(5):820-824. |
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| Authors: | WANG Sha-sha HE Jin-hua LUO Shi-nian PENG Feng LUO Lin HUANG Bao-ling LYU Cheng-qun |
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| Abstract: | Objective]Influence of Entomogenous Pestalotiopsis disseminata on nutrients metabolism of Hemiberlesia pitysophila Takagi was studied, in order to provide reference for mechanism of Entomogenous P. disseminata causing H. pitysophila Takagi dead and using Entomogenous P. disseminata to control H. pitysophila Takagi. Method]Entomogenous P. disseminata GXSTYJ03 strain was utilized to make the spore suspension of 1×107 spores/mL and infected H. pitysophila Takagi. Spraying sterile Tween was taken as control(CK) Contents of trehalose, glucose, protein and trehalase activity in H. pitysophila Takagi were detected for eight consecutive days. Result]At all measurement times, trehalose content of treatment group were lower than that of CK group, as treatment time extended, trehalose content of treatment group de-clined and was significantly lower than that of CK group 3 d after infection(P<0.05, the same below). At all measurement times, trehalase activity of treatment group were higher than that of CK group, and was significantly higher on day 3 of infection and 5 d after infection. Glucose content increased rapidly 1-3 d after infection and reached the peak (3.1479 mmol/L) on day 3, then declined gradually, and the difference with CK group was insignificant on 4-6 d(P>0.05), and significantly lower than that of CK group after 6 d. Protein content of treatment group was lower than that of CK group at all measurement times, and was at significant or extremely significant level(P<0.01) 2 d after infection. Conclusion]When Entomogenous P. disseminata GXSTYJ03 strain infecting H. pitysophila Takagi, trehalose, glucose and protein content decrease sharply, which is one of the lethal reasons. |
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| Keywords: | Entomogenous Pestalotiopsis disseminata Hemiberlesia pitysophila Takagi trehalose glucose treha-lase protein |
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